摘要目的 通过研究美沙酮对小鼠肠道菌群的影响,探讨美沙酮与机体疾病发生的关系。 方法 将75只小鼠随机分为三组,分别用美沙酮、燕麦β-葡聚糖和生理盐水进行灌胃,28 d后收集小鼠粪便,提取细菌基因组DNA,应用实时荧光定量PCR法对6种细菌进行定量。 结果 美沙酮组总肠道菌群(9.93±0.16 vs. 9.78±0.17)、拟杆菌属(8.84±0.41 vs. 8.46±0.54)、大肠杆菌(7.08±0.21 vs. 6.86±0.05)数量均显著高于生理盐水组(P<0.05)。 结论 美沙酮对小鼠肠道菌群有一定影响,能使小鼠粪便中总肠道菌群、拟杆菌属、大肠杆菌数量增加,肠道微生物定植抗力减弱。
Abstract：Objective To explore the relationship between methadone and the occurrence of diseases by studying the impact of methadone on intestinal flora in mice. Methods Seventy-five mice were randomly divided into three groups, and then the three groups were intragastrically administered with methadone, oat β-glucan and normal saline respectively. Mouse faeces were sampled to extract bacterial genome DNA 28 days later, and then real-time fluorescence quantitative PCR was employed to detect the amounts of 6 kinds of bacteria. Results The total intestinal flora(9.93±0.16 vs. 9.78±0.17), Bacteroides(8.84±0.41 vs. 8.46±0.54) and Escherichia coli counts(7.08±0.21 vs. 6.86±0.05)were all significantly higher in the methadone group than in the normal saline group (all P<0.05). Conclusions Methadone has certain influence on the intestinal flora in mice, and it can increase the total intestinal flora, Bacteroides and Escherichia coli counts in the feces of mice, and decrease the intestinal microbial colonization resistance.
黎勇, 申元英, 闵梦雅, 邓琦蕾, 张敏. 美沙酮对小鼠肠道菌群的影响[J]. 实用预防医学, 2018, 25(5): 524-528.
LI Yong, SHEN Yuan-ying, MIN Meng-ya, DENG Qi-lei, ZHANG Min. Effect of methadone on intestinal flora in mice. , 2018, 25(5): 524-528.
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