Association analysis of polymorphisms in OPRM1 gene and methadone maintenance treatment dose
LUO Xi1, LI Xingli1, HU Peiwu2, LUO Rui1, LIU Zhisheng1
1.Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China; 2. Department of Scientific Research, Xiangya Hospital ,Central South University, Changsha 410008;
Abstract：Objective To explore the association between the single nucleotide polymorphism (SNP) of the μ opioid receptor gene and methadone maintenance treatment dose. Methods The genotypes of the six SNPs (rs6912029, rs510769, rs3798676, rs7748401, rs589046, rs2075572) in 696 patients of methadone maintenance treatment clinics in Han Chinese population were detected by Multiple PCR technology, MassARRAY iPLEX single-base extension technology and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) SNP genotyping technique. The past drug use and treatment information were collected by questionnaires. The association between methadone dose and the two SNPs was analyzed. Result The frequencies of genotype, allele and haplotype at six SNPs (rs6912029,rs510769,rs3798676,rs7748401,rs589046,rs2075572) were not significantly different between the high dose and the low dose groups. Conclusion There may be no significant relevance between the OPRM1 gene SNPs (rs6912029 rs510769 rs3798676, rs7748401, rs589046, rs2075572) and methadone maintenance treatment dose of heroin There may be no significant relevance between the OPRM1 gene SNPs (rs6912029, rs510769, rs3798676, rs7748401, rs589046, rs2075572) and methadone maintenance treatment dose of heroin abusers.
罗希, 李杏莉, 胡佩武, 罗芮, 刘志胜. μ阿片受体基因多态性与美沙酮维持治疗剂量的关联分析[J]. 实用预防医学, 2016, 23(5): 513-515.
LUO Xi, LI Xingli, HU Peiwu, LUO Rui, LIU Zhisheng. Association analysis of polymorphisms in OPRM1 gene and methadone maintenance treatment dose. , 2016, 23(5): 513-515.
[1] Lobmaier P, Gossop M, Waal H, et al. The pharmacological treatment of opioid addiction—a clinical perspective[J]. European Journal of Clinical Pharmacology,2010,66(6):537-545. [2] 白延智,张宪武. 海洛因依赖的危害及美沙酮维持治疗概述[J]. 内蒙古医科大学学报,2014(S1):167-170. [3] 石珊,黄映善,李欣,等. 影响美沙酮维持治疗效果的相关因素分析[J]. 中国药物依赖性杂志,2008(01):56-61. [4] Baumeister M, Vogel M, Dursteler-Macfarland K M, et al. Association between methadone dose and concomitant cocaine use in methadone maintenance treatment: a register-based study[J]. Subst Abuse Treat Prev Policy,2014,9:46. [5] Al-Eitan L N, Jaradat S A, Su S Y, et al. Mu opioid receptor (OPRM1) as a predictor of treatment outcome in opiate-dependent individuals of Arab descent[J]. Pharmgenomics Pers Med,2012,5:99-111. [6] 张存敏. μ阿片受体基因第一外显子区和5’调控区单核苷酸多态性与海洛因依赖的相关性研究[D]. 昆明医学院,2007. [7] 率银良,熊晓燕,孙艳,等. 北京地区美沙酮维持治疗患者脱失原因调查[J]. 中国药物依赖性杂志,2010,19(4):277-280. [8] 谢小虎,刘惠芬,张亚海,等. μ阿片受体基因内含子2多态性与美沙酮维持治疗剂量的关联分析[J]. 中国临床药理学与治疗学,2013(10):1081-1085. [9] 刘小利,王陆瑶,胡文祥. μ阿片受体的三维结构预测及活性位点分析[J]. 化学通报,2009(02):133-137. [10] Wang S, Tsou H, Ho I, et al. Pharmacogenomics study in a Taiwanese methadone maintenance cohort[J]. Journal of Food and Drug Analysis,2013,21(4):S62-S68. [11] Levran O, Peles E, Randesi M, et al. Association of genetic variation in pharmacodynamic factors with methadone dose required for effective treatment of opioid addiction[J]. Pharmacogenomics,2013,14(7):755-768. [12] 谢小虎,庄丁丁,张亚海,等. 海洛因成瘾者美沙酮维持治疗剂量的调查分析[J]. 中国药物滥用防治杂志,2011(06):314-316.
2016, Vol. 23 
