Abstract：Objective To explore the alteration of gut microbiota in the disease state by 16s rRNA sequencing analysis of intestinal microbial population in rats with diabetes-related depression (DD). Methods A DD model was established, and healthy SD rats were used as the blank control group. The rats were weighed weekly, and fasting blood glucose was measured simultaneously. After the model was established, the rats’ behavior was examined by forced swimming test and open field test. The intestinal contents of rats in each group were collected, and 16s rRNA sequencing was performed and analyzed. The serum contents of hypothalamic-pituitary-adrenal (HPA) axis-related proteins and lipopolysaccharide (LPS) in rats of each group were detected. Results Compared with the blank control group, the DD model group showed significantly higher blood glucose and exhibited significant depression-like behavior and HPA axis hyperactivity. 16s rRNA sequencing results displayed that compared with the blank control group, α diversities (Chao, Simpson and Shannon indexes) of gut microbiota in the DD model group were not significantly different, but β diversity was significantly different, and the distance between samples in the blank control group and the DD model group was far. The gut microbiota differed significantly between groups. The abundance of phylumFirmicutes in the DD model group was significantly lower compared with the blank control group (P<0.01), while phylumBacteroidetes was not significantly different. Bugbase phenotypic prediction analysis showed significantly higher gram-negative bacteria and higher serum level of LPS in the DD model group compared with the blank control group. Conclusion The gut microbiota in the DD model group is disorderly distributed, with changes different from those in diabetes or depression alone. Gut microbiota disturbance leads to an increase in conditionally pathogenic bacteria in DD and thus results in a rise in LPS, which may be related to HPA axis hyperactivity.
马文恺, 李薇, 雷诗卉, 杨蕙, 王瑾茜. 糖尿病并发抑郁症大鼠肠道菌群失衡初步研究[J]. 实用预防医学, 2023, 30(2): 139-143.
MA Wen-kai, LI Wei, LEI Shi-hui, YANG Hui, WANG Jin-xi. Preliminary study on the imbalance of gut microbiota in rats with diabetes mellitus complicated by depression. , 2023, 30(2): 139-143.
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2023, Vol. 30 
