Abstract：Objective A confirmative method was developed to detect 14 β-lactam antibiotics residues in human urine by ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). Methods Urine samples were analyzed following incubation with β-glucuronidase, and then purified with an Oasis PRiME HLB (200 mg,6 ml) solid-phase extraction (SPE) cartridge. The UPLC separation was performed on an Waters BEH C18 column (2.1×100 mm, 1.7 μm) utilizing a gradient elution program of methanol (containing 0.01 mol/L ammonium acetate) and water (containing 0.01 mol/L ammonium acetate) as the mobile phase. Identification and quantification were achieved using electrospray ionization (ESI) in positive mode with multiple reaction monitoring (MRM). Results Good linearities of amoxicillin and ceftriaxone were achieved over the concentration of 10-1,000 ng/ml, and other 12 analytes were 5-1,000 ng/ml, with the correlation coefficients being above 0.99. The method quantitation limits (MQL) for amoxicillin and ceftriaxone were 10 ng/ml, and for other target compounds were 5 ng/ml. The detection limits of the method were 0.5-2.9 ng/ml. Average recoveries of analytes at three spiked levels ranged from 79.8% to 97.9%, with relative standard deviations (RSDs) of 2.1%-11.9%. Conclusion This method is of simple operation, high sensitivity, specificity and precision, and it is applicable to the determination of 14 β-lactam antibiotics residues in human urine.
李宏亮, 闵巍, 郑磊, 詹铭, 郝莉鹏. 超高效液相色谱-串联质谱测定尿中14种β-内酰胺类抗生素残留[J]. 实用预防医学, 2021, 28(10): 1194-1197.
LI Hong-liang, MIN Wei, ZHENG Lei, ZHAN Ming, HAO Li-peng. Determination of 14 β-lactam antibiotics residues in human urine by ultra performance liquid chromatography coupled with tandem mass spectrometry. , 2021, 28(10): 1194-1197.
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2021, Vol. 28 
