Abstract：Objective The fat deposition of kidneys of rabbits in each group was compared and the abnormal genes related to fat metabolism were screened by constructing the rabbit models of diabetes mellitus, syphilis and diabetes mellitus combined with syphilis. Methods The rabbit models of diabetes mellitus (the DM group) were established by injecting alloxan in ear vein, the rabbit models of syphilis (the Tp group) by vaccinating Treponema pallidum (T. pallidum) in testis, and the rabbit models of diabetes mellitus combined with syphilis (the H group) by injecting alloxan before vaccinating T. pallidum. Nitrogen (BUN) and creatinine(Cr) were detected by ear vein blood at the 2nd, 4th, 6th, 8th, 10th and 14th weeks respectively. After 14 weeks, fatty deposits of kidneys of the rabbits in each group were detecteded by oil red O staining and HE staining. Abnormal adipose metabolism gene candidates in renal tissues were screened by gene chip technique. Results The diabetes mellitus rabbit models, the syphilis rabbit models and the diabetes mellitus combined with syphilis models were constructed successfully. Renal function damage was found in the rabbits of each experimental group, and it was getting worse as the disease progression. Compared with other groups, the H group showed the severest renal function damage. The Results from oil red O staining showed that lipid droplet deposition in renal tissues of the H group was the most intensive. The Results from gene chip detection indicated that ACOX1, ACADM and SCP2 genes in adipose metabolism of rabbits in experimental groups showed statistically significant differences. The expression of the above-mentioned three genes in the groups of Tp, DM and H was down-regulated as compared with the normal group, and the most significant down-regulation was found in the H group. Conclusions The rabbit models of diabetes mellitus combined with syphilis (the H group) show the severest renal damage and fat deposition. The down-regulation of ACOX1, ACADM and SCP2 expression in the experimental rabbits may be related to fat deposition.
易婷, 曾丹, 刘炀, 刘爱胜, 李嘉怡, 谢小平, 刘双全. 糖尿病合并梅毒兔肾脂肪变相关基因的芯片筛选[J]. 实用预防医学, 2018, 25(5): 520-523.
YI Ting, ZENG Dan, LIU Yang, LIU Ai-sheng, LI Jia-yi, XIE Xiao-ping, LIU Shuang-quan. Microassay screening of renal fat deposition-related genes in diabetic rabbits with syphilis. , 2018, 25(5): 520-523.
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2018, Vol. 25 
