Detection of eight kinds of sugars in health foods by liquid chromatography with evaporative light scattering detector
YUAN Peng1, LI Rui2, YANG Rui-chun1
1. Henan Provincial Center for Disease Control and Prevention, Zhengzhou, Henan 450016, China; 2. Henan University of Chinese Medicine, Zhengzhou, Henan 450046, China
Abstract:Objective To develop a high performance liquid chromatography (HPLC) method with evaporative light scattering detector (ELSD) for detecting eight kinds of sugars in health foods. Methods Eight kinds of sugars (including xylose, fructose, sorbol, glucose, sucrose, maltose, lactose and raffinose) from health foods were extracted with solvent by ultrasonic-assisted heating. Analytes were separated by using a NH2P-50-4E chromatographic column with acetonitrile and 0.1% triethylamine aqueous solution as mobile phase, and then detected with evaporative light scattering detector. Results The peak area logarithmwas linearly related to the concentration logarithm of the sugars in the range of 5.0-400.0 mg/L. The range of minimum detectable mass concentration was 5.0 mg/L-20 mg/L. The detection precision was less than 1.5%, and the recoveries were 86.7%-104.5%. Conclusions The developed HPLC-ELSD method is suitable for determining the content of the sugars in health foods with the advantages of simple operation, strong anti-interference capability and quantitative accuracy.
袁鹏, 李蕊, 杨瑞春. 液相色谱-蒸发光散射检测法测定保健食品中8种糖的含量[J]. 实用预防医学, 2020, 27(9): 1092-1095.
YUAN Peng, LI Rui, YANG Rui-chun. Detection of eight kinds of sugars in health foods by liquid chromatography with evaporative light scattering detector. , 2020, 27(9): 1092-1095.