Abstract:Objective To explore the expression and value of CTLA-4 in peripheral blood lymphocytes of children with systematic lupus erythematosus (SLE). Methods Thirty children with SLE hospitalized in Department of Pediatric Nephrology, Qingdao Women and Children’s Hospital from January 2012 to September 2015 were randomly selected as SLE group, including 16 cases of active SLE and 14 cases of stationary SLE. 30 healthy children were simultaneously selected as the control group. The expression levels of CD28 and CTLA-4 in peripheral blood T lymphocytes of the 2 groups were tested by flow cytometry. Results No statistically significant difference was found in the expression of CD28 in peripheral blood CD3+, CD4+ and CD8+ T cells between SLE group and the control group (all P>0.05). The expression levels of CTLA-4 in peripheral blood CD3+, CD4+ and CD8+ T cells of SLE group were all low ((0.92±0.53), (0.55±0.41), (0.57±0.29)). The expression of CTLA-4 molecule in peripheral blood CD3+ and CD4+ T cells of SLE group was significantly lower than that of the control group (t=3.06, t=3.11, P<0.05), and the expression of CTLA-4 molecule in CD8+ T cells was also lower than that of the control group, but no statistically significant difference was found (t=0.84, P>0.05). The expression of CTLA-4 molecule in peripheral blood CD3+ and CD4+ T cells of active SLE group ((0.74±0.47), (0.41±0.24)) was significantly lower than that of the control group (t=3.40, t=4.48, both P<0.05), and the expression of CTLA-4 molecule in CD8+ T cells (0.47±0.29) was also lower than that of the control group (0.65±0.43), but there was no statistically significant difference (t=1.50, P>0.05). The expression levels of CTLA-4 in peripheral blood CD3+, CD4+ and CD8+ T cells of stationary SLE group were all higher than those of active SLE group, but there was no statistically significant difference compared with the control group (all P>0.05). Conclusions There exists a defective expression of CTLA-4 molecule in the peripheral blood T cells from children with SLE; moreover, it is involved in the pathogenesis of SLE. The expression of CTLA-4 is significantly decreased in the pathogenesis and progress of SLE, which induces an active immune response and further development of the disease.
王一冰, 崔广梅, 封东宁, 孙莉莉, 孙清. 儿童系统性红斑狼疮外周血淋巴细胞表面CTLA-4的表达及意义[J]. 实用预防医学, 2016, 23(11): 1381-1384.
WANG Yi-bing, CUI Guang-mei, FENG Dong-ning, SUN Li-li, SUN Qing. Expression and significance of CTLA-4 in peripheral blood lymphocytes of children with systematic lupus erythematosus. , 2016, 23(11): 1381-1384.
[1] Feng JB,Ni JD,Yao X,et al.Gender and age influence on clinical and laboratory features in Chinese patients with systemic lupus erythematosus:1790 cases[J].Rheumatol Int,2010,30(8):1017-1023. [2] Kamphuis S,Silverman ED.Prevalence and burden of pediatric-onset systemic lupus erythematosus[J].Nat Rev Rheumatol,2010,6(9):538-546. [3] Chen Z,Zhou F,Huang S,et al.Association of cytotoxic T lymphocyte associated antigen-4 gene ( rs60872763 )polymorphism with Crohn's disease and high levels of serum sCTLA-4in Crohn’s disease[J].J Gastroenterol Hepatol,2011,26(5):924-930. [4] Erfani N,Razmkhah M,Ghaderi A.Circulating soluble CTLA4( sCTLA4)is elevated in patients with breast cancer[J].Cancer Invest,2010,28(8):828-832. [5] Bonmbardier C,Gladman DD,Urowiz MB,et al. Derivation of the SLEDAI:a diease activity index for lupus patients[J].Arthritis Reum,1992,35(4):630-640. [6] 于清,薛海燕,曹兰芳.104 例儿童系统性红斑狼疮临床分析[J].临床儿科杂志, 2011,29(10):942-947. [7] 郭雨凡,孙凌云,邹耀红,等.1958 例系统性红斑狼疮住院患者首发表现和起病特点的回顾分析[J].中华风湿病学杂志,2011,15(2):105-107. [8] Tang Y,Chen Y,Ni B,et al.Up-regulation of the expression of costimulatory molecule CD40 in hepatocytes by hepatitis B virus X antigen[J].Biochem Biophys Res Commun,2009,384(1):12-17. [9] 王淑琼,罗玮,姚勇利.青海汉族Graves病甲状腺肿大与CTLA-4基因多态性相关性研究[J].实用预防医学,2015,10(9):1136-1138. [10] 周树录,李幼姬,叶任高.SLE患者Th1/Th2平衡状态及与外周血淋巴细胞CD28及CTLA-4分子表达的关系[J].中国病理生理杂志,2010, 26(8):1610-1615. [11] 章圣辉,毕来喜,韩义香,等.CD28/CT LA-4 :B7 在特发性血小板减少性紫癜患者外周血淋巴细胞中的表达及意义[J].临床血液学杂志,2010,23(1):77-79. [12] da Silva AE, dos Reis-Neto ET, da Silva NP, et al.The effect of acute physical exercise on cytokine levels in patients with systemic lupus erythematosus[J].Lupus, 2013, 22(14):1479-1483.