Correlation of single nucleotide polymorphism of XRCC1 with effects of platinum-based chemotherapy on peripheral blood cell parameters in patients with non-small cell lung cancer
WANG Xiao-wei*, CHEN Jian-hua, DENG Hong-yu, LIU Bo, ZHAO Jin, XIAO Ping, LIU Ting
*Hunan Cancer Hospital & the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, China
Abstract：Objective To explore the relationship between single nucleotide polymorphism of X-ray repair cross complementary gene 1 (XRCC1) and the changes of peripheral blood cell parameters before and after platinum-based chemotherapy in patients with non-small cell lung cancer (SNCLC). Methods Peripheral blood samples of 54 patients with NSCLC were collected before the chemotherapy, and then the polymorphisms of Arg194Trp and Arg399GIn genotypes in the XRCC1 were detected using the pyrosequencing assays. The changes of peripheral blood cell counts were observed before and after platinum-based chemotherapy. Results There were statistically significant differences in the counts of white blood cell, neutrophil, lymphocyte, red blood cell, platelet and platelet hematocrit in patients with the XRCC1 194Arg/Trp and Trp/Trp genotypes between before and 7, 10 and 13 days after the chemotherapy (P<0.05). There were statistically significant differences in the counts of white blood cell, neutrophil, monocyte, lymphocyte, red blood cell, platelet and platelet hematocrit in patients with the XRCC1 399 GIn/GIn genotype between before and 7, 10 and 13 days after the chemotherapy (P<0.05). Only the lymphocyte count in patients with the XRCC1 194Arg/Arg genotype showed a statistically significant difference between before and 7, 10 and 13 days after the chemotherapy (P<0.05). No statistically significant difference was found in the peripheral blood cell count of patients with the XRCC1 399Arg/GIn+Arg/Arg genotype before and after the chemotherapy (P>0.05). Conclusions Platinum-based chemotherapy mostly affects the peripheral blood cell count in patients with the XRCC1 399GIn/GIn genotype, followed by patients with the XRCC1 194Arg/Trp+Trp/Trp genotypes. Only a decrease in the lymphocyte count is found in patients with the XRCC1 194 Arg/Arg genotype. The decrease of peripheral blood cell count in patients with XRCC1 399Arg/GIn+Arg/Arg genotypes is not correlated with platinum-based chemotherapy. The clinicians may be able to predict the toxic and side effects of platinum-based drugs through detecting the polymorphism of the XRCC1 gene.
王小卫,陈建华, 邓红玉, 刘波,赵瑾,肖平,刘婷. XRCC1 单核苷酸多态性与铂类药物化疗对肺癌患者外周血细胞参数影响的相关性分析[J]. 实用预防医学, 2016, 23(11): 1306-1309.
WANG Xiao-wei, CHEN Jian-hua, DENG Hong-yu, LIU Bo, ZHAO Jin, XIAO Ping, LIU Ting. Correlation of single nucleotide polymorphism of XRCC1 with effects of platinum-based chemotherapy on peripheral blood cell parameters in patients with non-small cell lung cancer. , 2016, 23(11): 1306-1309.
2016, Vol. 23 
