Relationship of expression of VEGF protein and frequency of VEGF-2578C/A allele with clinical characteristics and prognosis of non-small cell lung cancer
CHEN Huan, WANG Wei, CHEN Guo-zhen
The PLA Navy Anqing Hospital, Anqing, Anhui 246003, China
Abstract：Objective To explore the relation of expression of vascular endothelial growth factor (VEGF) protein and frequency of VEGF-2578C/A allele with the clinical features and prognosis of non-small cell lung cancer (NSCLC). Methods We enrolled 80 patients with NSCLC hospitalized in the PLA Navy Anqing Hospital from March 2018 to February 2020, and collected their clinical data, including gender, age, history of smoking, pathological type, clinical stage, lymph node metastasis, family history, surgical method, chemotherapy cycle, and chemotherapy regimen. The expression of VEGF protein in cancer tissues was determined by immunohistochemistry, and the distribution of VEGF-2578C/A alleles was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The expression of VEGF protein, the frequency of VEGF-2578C/A allele, and the relationship between paracancerous tissue and the clinical characteristics of the patients were analyzed. The patients were followed up for 2 years, and then divided into the survival group and the death group according to their prognoses. The expression of VEGF protein and the distribution of VEGF-2578C/A alleles in the two groups were compared. Results Among the 80 patients, 49 (61.25%) were positive for VEGF, and 10 (12.50%) positive for paracancerous tissue. Patients positive for VEGF were in clinical stage Ⅲ-Ⅳ, and lymph node metastasis was significantly higher in patients positive for VEGF than in ones negative for VEGF (P<0.05). Among the 80 patients, the frequency of VEGF-2578C/A allele showed that there were 49 (61.25%) patients with CC genotype, 6 (7.50%) ones with AA genotype, and 25 (31.25%) ones with CA genotype. The proportions of clinical stage Ⅲ-Ⅳ and lymph node metastasis were significantly higher in patients with CC genotype than in ones with CA genotype (both P<0.05). 2-year telephone follow-up indicated that 56 (70.00%) patients survived and 24 (30.00%) died. The proportions of positive VEGF and VEGF-2578C/A allele/CC genotype were significantly higher in the death group than in the survival group (both P<0.05). Conclusion Positive expression of VEGF protein was prevailing in NSCLC patients with clinical stage Ⅲ-Ⅳ and lymph node metastasis. CC genotype was most common in VEGF-2578C/A allele; moreover, patients with positive expression of VEGF protein and CC genotype showed poorer prognoses.
陈焕, 王伟, 陈郭珍. VEGF蛋白表达及VEGF-2578C/A等位基因频率与非小细胞肺癌临床特征、预后的关系[J]. 实用预防医学, 2021, 28(8): 922-925.
CHEN Huan, WANG Wei, CHEN Guo-zhen. Relationship of expression of VEGF protein and frequency of VEGF-2578C/A allele with clinical characteristics and prognosis of non-small cell lung cancer. , 2021, 28(8): 922-925.
2021, Vol. 28 
