Abstract：Objective To explore the practicability of detecting the single nucleotide polymorphism (SNP) of tumor necrosis factor-alpha (TNF-α) rs1800629 in Uygur patients with vitiligo by high resolution melting (HRM) assay. Methods HRM assay was employed to detect the SNP of rs1800629 site in promoter region of TNF-α in 100 Uygur patients with vitiligo, and the detection results were analyzed and compared with the direct sequencing results of polymerase chain reaction (PCR) products. Results The sensitivity of rs1800629 SNP could reach 5% by HRM assay. The results of HRM assay were consistent with those of the direct sequencing of PCR products, with the coincidence rate of 100%. Conclusions HRM assay has a high sensitivity and accuracy, and it is a rapid, simple, high-throughput and optimal method for the detection of TNF-α SNP in patients with vitiligo.
赵宗峰,吴秀娟,吴卫东,普雄明,康晓静. 建立高分辨率熔解曲线法检测白癜风患者 TNF-α rs1800629基因多态性[J]. 实用预防医学, 2016, 23(11): 1302-1305.
ZHAO Zong-feng, WU Xiu-juan, WU Wei-dong, PU Xiong-ming, KANG Xiao-jing. Development of high resolution melting assay for detecting single nucleotide polymorphism of TNF-α rs 1800629 in patients with vitiligo. , 2016, 23(11): 1302-1305.
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