缺氧诱导因子1α调控人精子凋亡的作用研究

doi:10.3969/j.issn.1006-3110.2024.04.002

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摘要目的探讨缺氧诱导因子1α(hypoxia- inducible factor 1 alpha, HIF-1α)调控人精子凋亡的作用及机制,为弱精症等疾病的诊疗提供新的理论依据。方法将成年人精液分为精子活力正常组和弱精症组,分别加入HIF-1α干扰剂氯化钴或鸟苷酸环化酶激活剂利非西呱(lificiguat, YC-1),并分别设置不加药物处理的对照组。用全自动计算机辅助精液质量分析系统检测各组精子活力(以前向运动率表示);用酶联免疫检测仪检测精子凋亡水平、顶体酶活性;蛋白质印迹法检测HIF-1α、Bcl-2及Bax水平。结果与正常对照组相比,氯化钴处理组精子凋亡水平、HIF-1α和Bax蛋白的表达水平上升,精子活力、顶体酶活性、Bcl-2下降。与弱精症对照组相比,YC-1处理组精子凋亡水平、Bax表达下降,精子活力、顶体酶活性、HIF-1α、Bcl-2的表达上升。结论 HIF-1α水平可影响精子凋亡和活力。

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	张殷嘉
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	何永华

关键词 ：缺氧诱导因子-1α, 精子, 凋亡, 活力, 弱精症

Abstract：Objective To explore the role and mechanism of hypoxia-inducible factor 1α (HIF-1α) in regulating apoptosis of human sperm, and to provide a novel theoretical basis for diagnosis and treatment of diseases such as oligozoospermia. Methods Adult semina were divided into the normal sperm vitality group and the oligozoospermia group. Sperms in the two groups were incubated with HIF-1α inhibitor cobalt chloride or lificiguat (YC-1) respectively, and control groups without drug treatment were set up respectively. The computer-assisted semen analysis system was used to detect the vitality (expressed as progressive motility rate) of sperms in each group. Enzyme-linked immunosorbent assay was applied to measuring the sperm apoptosis level and acrosin activity. Western blotting was performed to detect the levels of HIF-1α, B-cell lymphoma-2 (Bcl-2) and Bcl2-associated X protein (Bax). Results Compared with the normal control group, the apoptosis rate and expression levels of HIF-1α and Bax in the cobalt chloride treatment group increased, but the sperm vitality, acrosin activity and expression level of Bcl-2 decreased. Compared with the oligozoospermia control group, the apoptosis level of sperms and expression level of Bax in the YC-1 treatment group decreased, but the sperm vitality, acrosin activity and expression levels of HIF-1α and Bcl-2 increased. Conclusion The level of HIF-1α can affect the sperm apoptosis and vitality.

Key words： hypoxia-inducible factor 1α sperm apoptosis vitality oligozoospermia

收稿日期: 2023-12-16

PACS:

R33

基金资助:广西自然科学基金项目(2020GxNsFDA297028)

通讯作者: 何永华,E-mail:hyhup@shmu.edu.cn。

作者简介: 张殷嘉(1999-),女,湖南长沙人,硕士研究生,研究方向:流行病学与卫生统计学。

引用本文:

张殷嘉, 胡婕, 莫林烽, 吴积维, 刘欣格, 何永华. 缺氧诱导因子1α调控人精子凋亡的作用研究[J]. 实用预防医学, 2024, 31(4): 389-392. ZHANG Yinjia, HU Jie, MO Linfeng, WU Jiwei, LIU Xinge, HE Yonghua. Regulating role of hypoxia-inducible factor 1α on apoptosis of human sperm. , 2024, 31(4): 389-392.

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https://www.syyfyx.com/CN/10.3969/j.issn.1006-3110.2024.04.002 或 https://www.syyfyx.com/CN/Y2024/V31/I4/389

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