Abstract：Objective To clarity the diagnosis of Brucella infection in suspected patients and diseased goats in Chenzhou City in 2015 from serological and molecular biological aspects. Methods An epidemiological survey was conducted among the patients. Blood culture was used to isolate and culture Brucella strains from the blood of the patients and goats with positive anti-Brucella antibody detected by the standard agglutination test, and then real-time fluorescence quantitative RT-PCR was employed for identification. The molecular biologicaland gene sequencing techniques were used to identify the species of the suspicious bacteria strains. Results In the serum antigen screening of 68 high-risk and suspected patients with brucellosis, 13 were detected to be positive. 57 of 1,679 serum samples from sick goats were detected to be positive, and 6 isolated strains were identified as Brucella meltensis by real-time fluorescence quantitative RT-PCR. Conclusions The main pathogen species causing brucellosis in Chenzhou City in 2015 is Brucella melitensis. Comprehensive measures should be taken to control its epidemic .
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2016, Vol. 23 
