Abstract:Objective To investigate the contamination status and pathogenic features of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province. Methods According to the aseptic sampling principle, 1,263 aquatic products of animal origin were collected from fair markets and supermarkets in 14 cities of Hunan Province. Vibrio parahaemolyticus was isolated and subjected to virulence gene detection, antimicrobial susceptibility testing and molecular typing by pulsed field gel electrophoresis (PFGE). Results Vibrio parahaemolyticus was detected in 221 of 1,263 aquatic products, with the detection rate of 17.50%. The trh gene was positive in 3 isolates and tdh gene in 1 isolate, with the virulence gene carrying rate of 1.81%. The isolates were the most resistant to ampicillin (30.32%), then to chloramphenicol (1.81%), sulfamethoxazole compound (1.36%) and tetracycline (0.45%). No multidrug resistance strain was detected. PFGE indicated that the bands of the isolates digested by Not I were relatively dispersed. Conclusions The contamination of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province is severe, and some isolates carry virulence gene and express resistance to antibiotics.
贾华云, 王岚, 陈帅, 张红, 梁进军, 刘晓革, 李俊华. 2010-2013年湖南省市售水产品中副溶血性弧菌污染状况及病原特征分析[J]. 实用预防医学, 2016, 23(12): 1433-1435.
JIA Hua-yun, WANG Lan, CHEN Shuai, ZHANG Hong, LIANG Jin-jun, LIU Xiao-ge, LI Jun-hua. Contamination status and pathogenic characteristics of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province, 2010-2013. , 2016, 23(12): 1433-1435.
[1] 裴晓燕,余波,张秀丽,等.中国内陆 6 省(自治区)淡水鱼养殖、 销售和餐饮环节常见嗜盐性弧菌污染调查[J].中国食品卫生杂志,2016,28(1):79-83. [2] 毛雪丹,胡俊峰,刘秀梅.2003-2007年中国1060起细菌性食源性疾病流行病学特征分析[J].中国食品卫生杂志,2010,22(3):224-228. [3] 李建国,石新时.邵阳市首次发现副溶血性弧菌食物中毒的调查报告[J].实用预防医学,2003,10(4):581-582. [4] Hirai H,Ito H, Hirayama T, et al.Molecular epidemiologic evidence for association of thermo stable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis[J] .Infect Immun, 1990, 58(11):3568-3573. [5] Raghunath P, Acharya S, Bhanumathi A, et al.Detection and molecular characterization of Vibrio parahaemolyticus isolated from seafood harvested along the southwest coast of India[J] .Food Microbiol, 2008, 25(6):824-830. [6] 胡世雄,邓志红,高立冬,等.湖南省O139霍乱流行因素及预防控制措施研究报告[J].实用预防医学,2010,17(4):651-653. [7] 吴蓓蓓,俞盈,金培婕,等.宁波地区海产品及环境中副溶血弧菌主要毒力及耐药性分析[J].中国人兽共患病学报,2011,27(5):381-385. [8] Han FF, Walker RD, Tanes ME, et al.Antimicrobial susceptibilities of Vibrio parahaemolyticus and Vibrio vulnificus isolates from louisiana gulf and retail raw oysters[J].App Environ Microb,2007, 73:7096-7098.