鼠伤寒沙门菌脉冲场凝胶电泳分子分型及耐药分析

doi:10.3969/j.issn.1006-3110.2023.08.008

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1075 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的收集2020—2021年间云南省的鼠伤寒沙门菌,了解其分子分型特征及耐药情况,为云南省鼠伤寒沙门菌防控提供参考。方法采用脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)方法对87株分离自云南省食源性疾病主动监测哨点医院感染患者的鼠伤寒沙门菌进行分型,应用BioNumerics7.6软件对酶切片段进行聚类分析,了解菌株的同源性特征;采用微量肉汤稀释法测定菌株对15种抗生素的药物敏感性。结果 87株鼠伤寒沙门菌分子分型呈65种PFGE带型,相似度区间为68.1％～100.0％,有一种优势带型;对氨苄西林的耐药率最高,达79.3%,其次为氯霉素(65.5%)、甲氧苄啶/磺胺甲噁唑(63.2%);对头孢西丁最为敏感,敏感率为98.9%,其次是阿奇霉素(95.4%)、多黏菌素E(95.4%);多重耐药率为79.4%,优势耐药谱是CHL+TET+AMP+AMS+CFZ+SXT。结论云南省鼠伤寒沙门菌PFGE型别既表现出明显的遗传多样性,也存在明显的优势带型,同时多重耐药情况较为严重。PFGE分子分型特征结合长期的药敏监测数据,可以协助分析菌株的耐药趋势,对指导临床用药有重要意义。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	王慧雯
	邹颜秋硕
	杨祖顺
	田云屏
	任翔

关键词 ：鼠伤寒沙门菌, 脉冲场凝胶电泳, 分子分型, 抗生素, 耐药

Abstract：Objective To collect Salmonella typhimurium strains in Yunnan Province from 2020 to 2021 for understanding their molecular typing characteristics and drug resistance, and to provide references for prevention and control of Salmonella typhimurium in Yunnan Province. Methods Eighty-seven strains of Salmonella typhimurium isolated from patients in foodborne disease active surveillance sentinel hospitals in Yunnan Province were typed by pulsed-field gel electrophoresis. BioNumerics7.6 software was used to cluster and analyze the digested fragments so as to understand the homology characteristics of the strains. The drugsensitivities of the strains to 15 antibiotics were determined by micro-broth dilution method. Results Molecular typing of the 87 strains of Salmonella typhimurium showed 65 PFGE bands, with a similarity range of 68.1%-100%, and there was a dominant band type. The resistance rate to ampicillin was the highest, reaching 79.3%, followed by chloramphenicol (65.5%) and trimethoprim/sulfamethoxazole (63.2%). The strains were most sensitive to cefoxitin with a sensitivity rate of 98.9%, followed by azithromycin (95.4%) and polymyxin E (95.4%). The multidrug resistance rate was 79.4%, and the dominant resistance spectrum was CHL+TET+AMP+AMS+CFZ+SXT. Conclusion PFGE typing of Salmonella typhimurium in Yunnan Province showed obvious genetic diversity as well as obvious dominant band. Meanwhile, the multidrug resistance was serious. The molecular typing characteristics of PFGE combined with long-term drug susceptibility monitoring data can assist in analyzing the drug resistance trend of the strains, which is of great significance for guiding clinical drug use.

Key words： Salmonella typhimurium pulsed-field gel electrophoresis molecular typing antibiotic drug resistance

收稿日期: 2022-08-20

R446.5

基金资助:云南省重大科技专项计划(202002AE320005)

通讯作者: 任翔 ,E-mail:kmrxhb@hotmail.com。

作者简介: 王慧雯(1987-), 女,学士,主管技师,研究方向:微生物检验。

引用本文:

王慧雯, 邹颜秋硕, 杨祖顺, 田云屏, 任翔. 鼠伤寒沙门菌脉冲场凝胶电泳分子分型及耐药分析[J]. 实用预防医学, 2023, 30(8): 932-935. WANG Huiwen, ZOU Yanqiushuo, YANG Zushun, TIAN Yunping, REN Xiang. Molecular typing by pulsed field gel electrophoresis and drug resistance of Salmonella typhimurium. , 2023, 30(8): 932-935.

链接本文:

https://www.syyfyx.com/CN/10.3969/j.issn.1006-3110.2023.08.008 或 https://www.syyfyx.com/CN/Y2023/V30/I8/932

[1] 许云敏,杜艳,单斌,等. 2005—2014年CHINET沙门菌属细菌耐药性监测[J]. 中国感染与化疗杂志,2016,16(3):294-301.
[2] 杨保伟. 食源性沙门氏菌特性及耐药机制研究[D].咸阳: 西北农林科技大学,2010.
[3] 魏琼,刘翔,张燕飞,等. 宁夏地区食源性与人源沙门菌耐药性与血清型对比研究[J].中国抗生素杂志,2016,41(9):707-709.
[4] 梁达炜,卢解红,江凌晓,等. 广州市136株沙门菌耐药表型与分子分型研究[J].现代预防医学,2016,43(4):696-699.
[5] Soyer Y,Alcaine SD, Schoonmaker-Bopp DJ,et al.Pulsed-field gel electrophoresis diversity of human and bovine clinical Salmonella isolates[J].Foodborne Pathog Dis,2010,7(6):707-717.
[6] 朱超, 许学斌. 沙门氏菌属血清型诊断[M]. 上海: 同济大学出版社,2009:1.
[7 ] Cheng MW,Lee CM,Wang NY,et al. Clinical characteristics in adult patients with Salmonella bacteremia and analysis of ciprofloxacin-nonsusceptible isolates[J]. J Microbiol Immunol Infect,2015,48(6):692-698.
[8] 沈赟,秦思,郑东宇,等. 江苏省2015—2019年沙门氏菌的血清型和耐药状况分析[J].现代预防医学,2021,48(12):2263-2267.
[9] 姚素霞,郝瑞娥,王洋,等. 2019—2020年山西省沙门菌耐药性及同源性研究分析[J]. 中国卫生检验杂志,2022,32(2):174-181.
[10] 曾献莹,吕素玲,杜悦,等. 2016年广西壮族自治区食源性沙门菌的耐药性与耐药谱研究[J]. 中国食品卫生杂志,2018,30(1):22-27.
[11] 耿英芝,于淼,李飞,等. 2016—2019年辽宁省肠炎沙门菌分离株分子分型及其耐药性[J]. 中国微生态学杂志,2021,33(7):770-773.
[12] 贾华云,王岚,张红,等. 食源性沙门菌耐药性研究进展[J]. 实用预防医学,2017,24(11):1401-1404.
[13] 杨柳青,欧新华,贾华云,等. 长沙市沙门菌表型特征及PFGE分子分型[J]. 实用预防医学,2016,23(1):39-42.
[14] 田云屏,邹颜秋硕,金莉,等. 云南省鼠伤寒沙门氏菌PFGE分子分型及耐药情况[J]. 昆明医科大学学报,2018,39(8):23-27.
[15] 杨劲松,陈建辉,林杰,等. 福建省鼠伤寒沙门菌PFGE分子分型和耐药的关联性研究[J].中国人兽共患病学报,2012,28(1):10-13.
[16] 赵嘉咏,黄丽莉,穆玉姣,等. 2011—2013年河南省鼠伤寒沙门菌耐药与分子分型研究[J]. 中国人兽共患病学报,2016,32(1):56-60.
[17] 苏良,杨柳青,宋迎春,等. 2015—2019年长沙市食源性疾病沙门菌血清分型和耐药情况分析[J]. 实用预防医学,2022,29(1):82-84.
[18] Ong G,Wilson I,Symth B,et al. Antimicrobial resistance in non-typhoidal salmonellas from humans in Northern Ireland,2001-2003: standardization needed for better epidemiological monitoring[J]. Epidemiol Infect,2007,135(4):675-680.
[19] Wei S,Chelliah R,Rubab M,et al. Bacteriophages as potential tools for detection and control of Salmonella spp.in food systems[J]. Microorganisms,2019,7(11):570.