发光菌评价3种药物与个人护理品的毒性研究

doi:10.3969/j.issn.1006-3110.2023.01.012

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摘要目的评价3种药物与个人护理品(pharmaceuticals and personal care products, PPCPs),即红霉素、乙酰螺旋霉素和邻苯二甲酸二丁酯对海洋发光菌的毒性。方法根据预试验的结果将红霉素、乙酰螺旋霉素和邻苯二甲酸二丁酯分别配制成不同浓度,以3% NaCl溶液作为空白对照,加入明亮发光杆菌T3变种,分别用生物毒性测试仪测试样品和空白对照的发光度,计算发光损失率,数据用SPSS 21.0软件进行统计学分析。结果样品发光度值分别随着红霉素、乙酰螺旋霉素和邻苯二甲酸二丁酯浓度的增加而降低,而发光损失率则分别随着3种PPCPs浓度的增加而增高,发光菌的发光损失率分别与3种PPCPs的浓度间呈线性回归关系(P＜0.001),拟合的红霉素毒性回归方程为Y=13.25+47.38X(R=0.933),乙酰螺旋霉素的毒性回归方程为Y=6.24+80.33X (R=0.956),邻苯二甲酸二丁酯毒性回归方程为Y=6.32+38.18X (R=0.952),3种PPCPs对发光菌的毒性各不相同,红霉素、乙酰螺旋霉素和邻苯二甲酸二丁酯的EC₅₀值分别为0.78×10^-3mol/L、0.54×10^-3mol/L和1.14×10^-5mol/L。结论利用发光菌对3种PPCPs进行毒性评价显示发光菌的发光抑制率与毒物浓度间呈较好的线性回归关系,通过EC₅₀值可以为判定3种PPCPs毒性强弱提供参考。

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关键词 ：药物与个人护理品, 红霉素, 乙酰螺旋霉素, 邻苯二甲酸二丁酯, 发光菌, 毒性

Abstract：Objective To evaluate the toxicity of three pharmaceuticals and personal care products (PPCPs), namely, erythromycin, acetylspiramycin and dibutyl phthalate to marine luminescent bacteria. Methods According to the results of the pre-experiment, erythromycin, acetylspiramycin and dibutyl phthalate were prepared into different concentration series respectively. At the same time, 3% NaCl solution was used as the blank control, and bright luminescent bacillus T3 was added. The luminosities of the samples and the blank control were tested by biological toxicity tester, and the luminescent loss rate was calculated. The data were statistically analyzed by SPSS 21.0 software. Results The luminosity of the samples decreased with the increase of concentration of erythromycin, acetylspiramycin and dibutyl phthalate, while the luminescent loss rate increased with the increase of concentration of the three PPCPs. The luminescent loss rate of luminescent bacteria showed a linear regression relationship with concentration of the three PPCPs (P<0.001). The toxicity regression equation of erythromycin was Y=13.25+47.38X(R=0.933), the toxicity regression equation of acetylspiramycin Y=6.24+80.33X(R=0.956), and the toxicity regression equation of dibutyl phthalate Y=6.32+38.18X(R=0.952). The toxicity of the three PPCPs to luminescent bacteria was different, and the EC₅₀ values of erythromycin, acetylspiramycin and dibutyl phthalate were 0.78×10^-3mol/L, 0.54×10^-3mol/L and 1.14×10^-5mol/L, respectively. Conclusion Toxicity evaluation of the three PPCPs by luminescent bacteria showed that there was a good linear regression relationship between the luminescence inhibition rate of luminescent bacteria and the concentration of poisons. TheEC₅₀ value can provide references for judging toxicity of the three PPCPs.

Key words： pharmaceuticals and personal care products erythromycin acetylspiramycin dibutyl phthalate luminescent bacteria toxicity

收稿日期: 2022-01-11

R123

基金资助:宁德师范学院2019年第二批引进人才科研启动项目(2019Y19);2019年宁德市指导性科技计划项目(20190015)

通讯作者: 陈思萍,E-mail: xuyd1827@sina.com。

作者简介: 张建江(1973-),男,博士,副教授,研究方向:水环境与健康。

引用本文:

张建江, 肖建忠, 陈思萍. 发光菌评价3种药物与个人护理品的毒性研究[J]. 实用预防医学, 2023, 30(1): 49-52. ZHANG Jian-jiang, XIAO Jian-zhong, CHEN Si-ping. Toxicity evaluation of three pharmaceuticals and personal care products on luminescent bacteria. , 2023, 30(1): 49-52.

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https://www.syyfyx.com/CN/10.3969/j.issn.1006-3110.2023.01.012 或 https://www.syyfyx.com/CN/Y2023/V30/I1/49

[1] Anim AK,Thompson K,Duodu GO,et al. Pharmaceuticals, personal care products, food additive and pesticides in surface waters from three Australian east coast estuaries (Sydney, Yarra and Brisbane)[J]. Mar Pollut Bull, 2020, 153(4):111014.
[2] Li Z,Yu X,Yu F,et al. Occurrence, sources and fate of pharmaceuticals and personal care products and artificial sweeteners in groundwater[J]. Environ Sci Pollut Res Int, 2021, 28(17):20903-20920.
[3] Lee HJ, Kim KY, Hamm SY, et al. Occurrence and distribution of pharmaceutical and personal care products, artificial sweeteners, and pesticides in groundwater from an agricultural area in Korea[J]. Sci Total Environ, 2019, 659:168-176.
[4] Xin X,Huang G,Zhang B. Review of aquatic toxicity of pharmaceuticals and personal care products to algae[J]. J Hazard Mater, 2020, 410(7):124619.
[5] 朱松梅,董玉瑛,沙志新,等. 大环内酯类抗生素对发光菌和脲酶活性影响分析[J]. 生态毒理学报,2020,15(3):116-122.
[6] Zhang H,Shi J,Su Y,et al. Acute toxicity evaluation of nanoparticles mixtures using luminescent bacteria[J]. Environ Monit Assess, 2020, 192(8):484.
[7] 杜丽娜,薛金玲,余若祯,等. 对《水质急性毒性的测定发光细菌法》的改进和探讨[J]. 中国环境监测,2013,29(6):123-127.
[8] 尹承南, 谢培, 焦萌, 等. 天津市地表水体54种PPCPs分布特征[J]. 环境化学, 2021, 40(9):2820-2831.
[9] Zhang Y, Lin L, Li Y, et al. Determination of 38 pharmaceuticals and personal care products in water by lyophilization combined with liquid chromatography-tandem mass spectrometry[J]. Anal Methods, 2021,13(3):299-310.
[10] 李建凤, 廖立敏. 含苯环化合物对发光菌急性毒性QSAR研究[J]. 环境科学与技术,2020,43(1):14-19.
[11] Muneeswaran T,Kalyanaraman N,Vennila T,et al. Rapid assessment of heavy metal toxicity using bioluminescent bacteria Photobacterium leiognathi strain GoMGm1[J]. Environ Monit Assess, 2021, 193(3):109.
[12] Li T,Li X,Liu H,et al. Preparation and characterization of molecularly imprinted polymers based on β-cyclodextrin-stabilized Pickering emulsion polymerization for selective recognition of erythromycin from river water and milk[J]. J Sep Sci, 2020, 43(18):3683-3690.
[13] 朱松梅,方政,董玉瑛. 3种大环内酯类抗生素对海洋发光菌的毒性作用[J]. 环境监控与预警, 2020, 12(5):112-116.
[14] 李思果,黄薇,姜杰,等. 深圳市市售酒类塑化剂检测结果分析与风险评估[J]. 实用预防医学,2019,26(9):1031-1034.
[15] Arzuaga X, Walker T, Yost EE, et al. Use of the Adverse Outcome Pathway (AOP) framework to evaluate species concordance and human relevance of dibutyl phthalate (DBP)-induced male reproductive toxicity[J]. Reprod Toxicol, 2020, 96:445-458.