2009—2019年上海市闵行区手足口病病原谱及EV-A71和CV-A16病毒分离株VP1区基因特征分析

doi:10.3969/j.issn.1006-3110.2022.10.002

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1255 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的了解2009—2019年上海市闵行区手足口病的病原谱,分析肠道病毒71型(enterovirus 71,EV-A71)和柯萨奇病毒A组16型(coxsackievirus A16,CV-A16)VP1区基因特征,为手足口病的综合防治提供科学依据。方法对2009—2019年闵行区手足口病监测点送检的标本应用实时荧光定量PCR进行病原学检测,分析病原学特征。EV-A71和CV-A16病毒分离株进行VP1区核苷酸序列测定,分析其同源性并构建系统进化树。结果 2009—2019年共收集到5 364例手足口病病例标本,病原学检测阳性检出率为86.74%(4 653/5 364),其中EV-A71检出率为40.12%(2 152/5 364)、CV-A16为16.61%(891/5 364)、CV-A6为20.86%(1 119/5 364)、CV-A10为1.49%(80/5 364)。2009—2014年主要呈EV-A71和CV-A16共同流行趋势,2015—2019年主要以CV-A6和CV-A16共同流行为主。EV-A71分离株均为C4a型,VP1区核苷酸序列同源性为92.1%~99.3%、氨基酸序列同源性为98.1%~100.0%; CV-A16分离株均为B1基因型,存在B1b和B1a基因亚型的共同流行,VP1区核苷酸序列同源性为87.2%~99.6%、氨基酸序列同源性为97.9%~100.0%。结论 2009—2019年上海市闵行区手足口病呈现EV-A71、CV-A16和CV-A6共同流行态势,不同年份的优势毒株呈现动态变化;而EV-A71流行株属于C4a亚型、CV-A16流行株存在B1b和B1a基因亚型的共同流行,与国内大部分地区的流行株基因亚型一致。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	闫红静
	甄珊珊
	付贝尔
	王之晗
	王小光
	张颖华

关键词 ：手足口病, 病原谱, 肠道病毒71 型, 柯萨奇病毒A组16 型, VP1基因

Abstract：Objective To understand the etiological spectrum of hand, foot and mouth disease (HFMD) and analyze the VP1 genetic characteristics of enterovirus A group 71 (EV-A71) and coxsackievirus group A 16 (CV-A16) in Minhang District of Shanghai from 2009 to 2019, and to provide a scientific basis for comprehensive prevention and treatment of HFMD. Methods Real time PCR was used to detect HFMD specimens from surveillance hospitals in Minhang District during 2009-2019, and the pathogenic characteristics were analyzed. VP1 region nucleotide sequences of EV-A71 and CV-A16 isolates were determined, the homology was analyzed and the phylogenetic tree constructed. Results A total of 5,364 specimens of HFMD cases were collected during 2009-2019. Etiological detection revealed that the positive detection rate was 86.74% (4,635/5,364), of which the detection rates of EV-A71, CV-A16, CV-A6 and CV-A10 were 40.12% (2,152/5,364), 16.61% (891/5,364), 20.86% (1,119/5,364) and 1.49% (80/5,364), respectively. It mainly showed the common epidemic trend of EV-A71 and CV-A16 during 2009-2014, while during 2015-2019 it was mainly the common epidemic trend of CV-A6 and CV-A16. EV-A71 isolates were all C4a sub-genotype, and the sequences analysis displayed that EV-A71 strains shared 92.1%-99.3% nucleotide similarity and 98.1%-100.0% amino acid similarity with each other. CV-A16 isolates were all B1 genotypes, and there was a common prevalence of B1b and B1a sub-genotypes, the sequences analysis revealed that CV-A16 strains shared 87.2%-99.6% nucleotide similarity and 97.9%-100.0% amino acid similarity with each other. Conclusion The prevalent pathogens of HFMD in Minhang District during 2009-2019 were EV-A71, CV-A16 and CV-A6, and the dominant strains in different years showed dynamic changes. The EV-A71 strains belonged to C4a sub-genotype, and the CV-A16 strains had the common prevalence of B1band B1a sub-genotypes. They were basically consistent with the sub-genotypes of epidemic strains in most parts of China.

Key words： hand, foot and mouth disease pathogenic spectrum enterovirus 71 coxsackievirus A16 VP1 gene

收稿日期: 2022-03-04

R725.1

基金资助:上海市闵行区自然科学研究课题(2019MIIZ011);闵行区公共卫生重点学科建设(MGWXK08)

通讯作者: 张颖华, E-mail: yhzhangcdc@sina.com。

作者简介: 闫红静(1987-),女,河南人,硕士,主管技师,研究方向:分子病毒。

引用本文:

闫红静, 甄珊珊, 付贝尔, 王之晗, 王小光, 张颖华. 2009—2019年上海市闵行区手足口病病原谱及EV-A71和CV-A16病毒分离株VP1区基因特征分析[J]. 实用预防医学, 2022, 29(10): 1158-1162. YAN Hong-jing, ZHEN Shan-shan, FU Bei-er, WANG Zhi-han, WANG Xiao-guang, ZHANG Ying-hua. Pathogenic spectrum of hand, foot and mouth disease and VP1 genetic characteristics of EV-A71 and CV-A16 in Minhang District, Shanghai, 2009-2019. , 2022, 29(10): 1158-1162.

链接本文:

https://www.syyfyx.com/CN/10.3969/j.issn.1006-3110.2022.10.002 或 https://www.syyfyx.com/CN/Y2022/V29/I10/1158

[1] Zhuang ZC, Kou ZQ, Bai YJ, et al. Epidemiological research on hand, foot, and mouth disease in mainland China[J]. Viruses, 2015, 7(12):6400-6411.
[2] 中华人民共和国卫生部.手足口病预防控制指南(2009年版)[M].北京:中华人民共和国卫生部,2009:5-6.
[3] 张正君,杨兴林,黄海.贵阳市 2013—2015年手足口病患儿EV71病毒VP1、VP4基因特征分析[J].中国公共卫生,2016,32(6):828-831.
[4] 黎俊宏,嵇红,樊欢,等.江苏省2015年柯萨奇病毒A组16型 VP1基因特征[J].病毒学报,2016,32(6):689-693.
[5] 赵博兰,崔立周,王春艳,等.2014—2019年保定市手足口病流行特征及病原学特征分析[J].实用预防医学,2021,28(7):877-880.
[6] 孙箐爽,董兆鹏,臧昊,等.2011—2018年上海市金山区手足口病病原学监测结果及柯萨奇病毒A6型分离株VP1基因特征分析[J].实用预防医学,2020,27(1):33-37.
[7] 段蓉,周毅彬,朱奕奕,等.2009—2019年上海市徐汇区HFMD 流行病学特征分析[J]. 职业与健康, 2021,34(14):1910-1915.
[8] Chang LY. Enterovirus 71 in Taiwan[J]. PEDN, 2008, 49(4):103-112.
[9] 史军,牛卫东,许太彬,等.2016—2020年郑州市儿童手足口病优势毒株变化趋势[J]. 河南预防医学杂志, 2021,32(8):645-647.
[10] 王希峰,周丽敏,郑天驰.2018—2019年北京市大兴区手足口病病原学特征及影响因素分析[J].中国现代医生,2020,58(22):143-147.
[11] 杨克江,许玲玉,李婷婷,等.2014—2018年昆明市官渡区手足口病流行特征分析[J].职业与健康,2020,36(12):1650-1654.
[12] 于宏杰,倪胜男,钟培松.上海市嘉定区2007—2016年手足口病流行特征及病原学分析[J].上海预防医学,2018,30(12):1005-1008.
[13] 张晓玲,俞慧菊,宋志刚,等.上海地区2011—2014年手足口病的病原谱变化及流行规律分析[J].上海预防医学,2018,30(5):380-385.
[14] 韩亦非,高洁,张久丁,等.2014—2018年汉中市手足口病病原学监测结果分析[J].职业与健康,2020,36(1):66-69.
[15] 王瑞琴,蔡旭,李飒,等.2018年北京市昌平区手足口病流行特征及经济负担分析[J].职业与健康,2020,36(18):2533-2536,2541.
[16] 高芳旭.2009—2018年天津市和平区手足口病流行特征分析[J].现代预防医学,2019, 46(18):3294-3296,3318.
[17] 吴燕飞,陈旭光,刘东妹,等.2017—2019年广东省 Cox A6 感染手足口病流行特征分析[J/OL].疾病监测.https://kns.cnki.net/kcms/detail/11.2928.R.20210928.1830.003.html.
[18] Oberste MS, Maber K, Kilpatrick DR, et al. Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification[J]. J Virol, 1999, 73(3):1941-1948.
[19] 谢群,谭徽,刘爱平,等. 2011年郴州市手足口病病原学及EV71 型分离株基因特征研究[J]. 实用预防医学,2013, 20(3): 291-297.
[20] Zhang Y, Wang D, Yan D, et al. Molecular evidence of persistent epidemic and evolution of subgenotype B1 coxsackievirus A16-associated hand, foot, and mouth disease in China[J]. J Clin Microbiol, 2010, 48(2):619-622.