2020年郑州某哨点医院儿童致泻性大肠埃希菌流行特征及分子分型分析

doi:10.3969/j.issn.1006-3110.2022.03.002

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (3053 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的了解郑州市某哨点医院儿童致泻性大肠埃希菌(diarrheagenic Escherichia coli, DEC)的流行特征及分子分型特征,为今后本地区科学防控儿童DEC感染提供依据。方法对2020年收集的230份腹泻儿童和187份非腹泻儿童粪便标本进行常规细菌培养,采用多重PCR技术检测DEC的毒力基因和脉冲场凝胶电泳(pulsed field gelelectrophoresis, PFGE)技术进行分子分型。结果 DEC在腹泻儿童与非腹泻儿童中的检出率分别为8.26%(19/230)和14.97%(28/187),腹泻儿童中检出的型别及检出率分别为典型黏附性大肠埃希菌(typical enteroaggregative Escherichia coli, tEAEC)(3.48%)、肠致病性大肠埃希菌(enteropathogenic Escherichia coli, EPEC)(2.61%)、非典型黏附性大肠埃希菌(atypical enteroaggregative Escherichia coli, aEAEC)(2.17%)和肠侵入性大肠埃希菌(enteroinvasive Escherichia coli, EIEC)(0.43%),非腹泻儿童中检出的型别为aEAEC(8.02%)、tEAEC(4.28%)、EPEC(3.74%)和产肠毒素大肠埃希菌(enterotoxigenic Escherichia coli, ETEC)(0.53%)。在腹泻儿童中黏液便的检出率高于稀便的检出率(χ²=47.332,P＜0.001),在不同性别、不同年龄段中检出率无显著性差异。46株DEC分为44个带型,种内最高相似度为100.00%,最低相似度为24.54%,腹泻儿童与非腹泻儿童之间并未检测出带型一致的条带。结论 DEC在郑州市腹泻儿童与非腹泻儿童中优势型别是tEAEC、aEAEC和EPEC,提示DEC为机会致病菌。常见DEC型别的PFGE指纹图谱呈高度多态性,腹泻儿童与非腹泻儿童未发现同源克隆菌株的感染。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	周鹏
	安戈
	李羿
	赵雪蕾
	张晓甍
	牛卫东

关键词 ：儿童, 致泻性大肠埃希菌, 多重PCR, 脉冲场凝胶电泳

Abstract：Objective To investigate the epidemic features and molecular typing of diarrheagenic Escherichia coli (DEC) from children in a sentinel hospital in Zhengzhou City, and to provide a basis for scientific prevention and control of DEC infection in children in future. Methods We collected 230 stool samples of diarrhea children and 187 stool samples of non-diarrhea children for routine bacterial culture in 2020. The virulence genes of DEC were detected by multiplex PCR, and pulsed field gel electrophoresis (PFGE) was used for molecular typing. Results The detection rates of DEC in the diarrhea and non-diarrhea children were 8.26% (19/230) and 14.97% (28/187), respectively. The most common pathogen in the diarrhea children was typical enteroaggregative Escherichia coli (tEAEC) (3.48%), followed by enteropathogenic Escherichia coli (EPEC) (2.61%), atypical enteroaggregative Escherichia coli (aEAEC) (2.17%) and enteroinvasive Escherichia coli (EIEC) (0.43%). The most common pathogen in the non-diarrhea children was aEAEC (8.02%), followed by tEAEC (4.28%), EPEC (3.74%) and ETEC (0.53%). Among the diarrhea children, the detection rate of DEC from mucus stool was higher than that from loose stool (χ²=47.332, P<0.001), and no statistically significant difference was found in the detection rate in different genders and different age groups. 46 strains of DEC were divided into 44 bands, with the highest similarity of 100% and the lowest similarity of 24.54%. No consistent bands were detected between the diarrhea children and the non-diarrhea children. Conclusion The dominant types of DEC in the diarrhea and non-diarrhea children in Zhengzhou City were tEAEC, aEAEC and EPEC, suggesting that DEC was an opportunistic pathogen. PFGE of the common types of DEC showed highly polymorphic, and no homologous clones were found between the diarrhea and non-diarrhea children.

Key words： children diarrhea Escherichia coli multiplex PCR pulsed filed gel electrophoresis

收稿日期: 2021-03-23

R725.7

基金资助:河南省细菌性传染病病原谱流行规律及变异研究(2018ZX10713003-002-003)

通讯作者: 安戈,E-mail:orangexxx@163.com。

作者简介: 周鹏(1987-),男,河南省新乡市人,硕士,主管技师,研究方向:微生物检验。

引用本文:

周鹏, 安戈, 李羿, 赵雪蕾, 张晓甍, 牛卫东. 2020年郑州某哨点医院儿童致泻性大肠埃希菌流行特征及分子分型分析[J]. 实用预防医学, 2022, 29(3): 261-264. ZHOU Peng, AN Ge, LI Yi, ZHAO Xue-lei, ZHANG Xiao-meng, NIU Wei-dong. Epidemic characteristics and molecular typing of diarrheagenic Escherichia coli from children in a sentinel hospital in Zhengzhou, 2020. , 2022, 29(3): 261-264.

链接本文:

https://www.syyfyx.com/CN/10.3969/j.issn.1006-3110.2022.03.002 或 https://www.syyfyx.com/CN/Y2022/V29/I3/261

[1] Yu J, Jing H, Lai S, et al. Etiology of diarrhea among children under the age five in China: results from a five-year surveillance[J]. J Infect, 2015, 71(1):19-27.
[2] Nataro JP,Kaper JB. Diarrheagenic Escherichia coli[J]. Clin Microbiol Rev, 1998, 11(1):142-201.
[3] Vijay D,Dhaka P,Vergis J,et al. Characterization and biofilm forming ability of diarrhoeagenic enteroaggregative Escherichia coli isolates recovered from human infants and young animals[J]. Comp Immunol Microbiol Infect Dis, 2015, 38: 21-31.
[4] 桑灏, 崔燕, 顾文超. 等. 2015—2018年上海市儿童腹泻流行特征及致泻性大肠埃希菌耐药分析[J]. 疾病监测, 2019, 34(6):559-564.
[5] 胡雪, 厉小玉, 周俊, 等. 杭州市腹泻儿童感染致泻性大肠埃希菌的病原学特征分析[J]. 中国卫生检验杂志, 2017, 27(22):3299-3300, 3318.
[6] 靳晓红, 马凤龙, 戚金荣, 等. 2014年济南市医院感染性腹泻常见病原菌检测与分析[J]. 实用预防医学, 2017, 24(1):65-67.
[7] 秦思, 沈赟, 马恺, 等. 2018—2019年江苏省食源性疾病中致泻大肠埃希氏菌流行特征及耐药性分析[J]. 现代预防医学, 2020, 47(21):3884-3888.
[8] 王维钧, 舒高林, 彭华, 等.北京市昌平区感染性腹泻标本常见病原菌谱分析[J]. 实用预防医学, 2019, 26(10):1274-1277.
[9] 吕虹, 黄伟峰, 黄玉兰, 等. 四川省腹泻患者和食品加工从业人员致泻性大肠埃希菌监测分析[J]. 中国卫生检验杂志, 2016, 26(8):1182-1184, 1202.
[10] 石挺丽, 黄建华, 李秀芬, 等. 2014—2015年广州地区腹泻患儿和健康儿童致泻性大肠埃希菌流行特征及耐药分析[J]. 中华疾病控制杂志, 2016, 20(4):329-332.
[11] 孙晖, 刘学通, 赵爱兰, 等. 肠集聚性大肠埃希菌分离株脉冲场凝胶电泳分析[J]. 疾病监测, 2013, 28(10):807-810.
[12] 胡玉琴, 章乐怡, 李毅, 等. 温州市沙门菌血清型、耐药性和PFGE指纹图谱研究[J]. 预防医学, 2019, 31(6):640-642.