Establishment of a rapid method for detecting influenza A virus
ZHU Yuan-ya1, DIAO Wei-jun2, HU Hong-zhang1
1. Chengdu Shang Jin Nan Fu Hospital, Chengdu, Sichuan 611743, China; 2. Maccura Biotechnology Co., Ltd., Chengdu, Sichuan 611731, China; 3. Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China
Abstract：Objective To establish a rapid and simple kit based on the colloidal gold immunochromatography assay (GICA) for the detection of human influenza A virus antigen. Methods Colloidal gold particles were prepared by the method of trisodium citrate reduction, and labeled with a monoclonal antibody against influenza A virus. The cellulose nitrate membrane was coated with another monoclonal antibody against influenza A virus and made into an immunochromatographic test strip. Influenza A viruses in the tested samples were firstly bound to colloidal gold labeled antibodies, and then moved to nitrocellulose membrane to react with fixed monoclonal antibodies and form a visible red band. Results The test strips could react specifically with different concentration of influenza A virus culture medium, and did not cross-react with 16 pathogens, such as influenza B virus, respiratory syncytial virus and parainfluenza virus. The sensitivities of the different concentration of the influenza A virus culture solution were the same as those of the similar products made in the USA. Stability detection displayed that 42℃ and 37℃ thermal destruction placement results were superior to those of the similar products made in the USA. Comparison and evaluation of clinical results showed that the Kappa value was 0.976, and the consistency was very high. Conclusions The sensitivity and specificity of the test strip can meet the requirement of clinical use; moreover, it has the advantages of simplicity and rapidness with no need for special apparatus and equipment. The test strip has important application value for the diagnosis and epidemiological investigation of influenza A.
2020, Vol. 27 
