Abstract:Object:This study is based on the latest report of 8 types of HPV genome in NCBI. The eight HPVs are HPV (126,135,136,137,140,144,154,166). the Phylogenetic tree was constructed, the boxshadow was built based on the L1 amino acid sequence and the specific primers was designed based on the L1 nucleotide sequence . Method:The similar HPV to 8 types was searched by Blast, the representative HPV of α, β , γ genus was selected, and the all of the 58 HPV L1 amino acid sequences was analyzed and compared by the Clustal W software to constructing the Phylogenetic tree. The specific primers were designed by the primer-blast. Result:From the Phylogenetic tree, we know that all the eight HPVs are from γ genu. Using the Primer-Blast, we designed the specific primers of each HPV. Conclusion:The HPV type of 126 ,135,136,137,140,144,154,166 are all in γ genus.
王启广, 谌芳, 曹友德. 8种新型HPV的分型及特异型引物设计[J]. 实用预防医学, 2016, 23(1): 111-114.
Wang Qiguang, Shen Fang, Cao Youde. Typing and the Primer design of eight new HPV. , 2016, 23(1): 111-114.
[1] Munoz N. Human papillomavirus and cancer: the epidemiological evidence[J]. Journal of clinical virology, 2000, 19(1): 1-5. [2] 阳月娥.716例妇科宫颈疾病患者HPV检测结果分析[J].实用预防医学,2013,20(8):992-993 [3] De Villiers E M, Fauquet C, Broker T R, et al. Classification of papillomaviruses[J]. Virology, 2004, 324(1): 17-27. [4] Holmes EC. The phylogeography of human viruses[J].Mol Ecol,2004,13(4):745-756. [5] 井明霞,陈继明,郭晓青.et al.人类乳头瘤病毒(HPV)的检测方法[J].现代预防医学, 2007, 22:4270- 4271. [6] 张友忠.HPV的检测方法及利弊[J].现代妇产科进展,2011,20(5):339-341. [7] Ye J, Coulouris G, Zaretskaya I, et al. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction[J]. BMC bioinformatics,2012,13(1):134. [8] Egawa N,Kawai K,Egawa K,et al. Molecular cloning and characterization of a novel human papillomavirus,HPV 126, isolated from a flat wart-like lesion with intracytoplasmic inclusion bodies and a peculiar distribution of Ki-67 and p53[J]. Virology,2012,422(1):99-104. [9] Bottalico D,Chen Z,Dunne A,et al. The oral cavity contains abundant known and novel human papillomaviruses from the Betapapillomavirus and Gammapapillomavirus genera[J]. Journal of Infectious Diseases,2011,204(5):787-792. [10] Li J, Cai H, Xu Z, et al. Nine complete genome sequences of cutaneous human papillomavir- us genotypes isolated from healthy skin of individuals living in rural he nan province, china[J]. Journal of virology,2012,86(21):11936-11936. [11] 洪蝶,陈怀增,谢幸,等.乳头瘤病毒分类进展[J].国外医学.病毒学分册,2005, 6:005. [12] Ault K A. Effect of prophylactic human papillomavirus L1 virus-like-particle vaccine on risk of cervical intraepithelial neoplasia grade 2,grade 3,and adenocarcinoma in situ: a combined analysis of four randomised clinical trials[J].The Lancet,2007,369(9576):1861-1868. [13] Paavonen J, Naud P, Salmeron J, et al. Efficacy of human papillomavirus (HPV)-16/18 AS04-adjuvanted vaccine against cervical infection and precancer caused by oncogenic HPV types (PATRICIA): final analysis of a double-blind, randomised study in young women[J].The Lancet,2009,374(9686):301-314. [14] Muñoz N, Kjaer S K, Sigurdsson K, et al. Impact of human papillomavirus (HPV)-6/11/16/18 vaccine on all HPV-associated genital diseases in young women[J].Journal of the National Cancer Institute,2010,102(5):325-339. [15] Munoz N, Bosch F X, Castellsagué X, et al. Against which human papillomavirus types shall we vaccinate and screen? The international perspective[J]. International Journal of Cancer,2004,111(2):278-285.