Determination of methanol in plasma by headspace gas chromatography-mass spectrometry
TANG Hua-min1,2, FU Sheng1, WANG An2
1. Xiangya School of Public Health, Central South University, Changsha, Hunan 410078, China; 2. Hunan Prevention and Treatment Institute for Occupational Diseases, Changsha, Hunan 410005, China
Abstract：Objective To establish aheadspace gas chromatography-mass spectrometry (HS-GC-MS/MS) method to determine methanol in plasma. Methods We designed a L9 (34) orthogonal experiment to optimize the process conditions of headspace. Samples were determined in the selected reaction monitoring mode, and external standard method was used in the quantification. Results Through the orthogonal experiment, the optimum headspace conditions were determined as follows: equilibrium temperature 80℃, equilibrium time 30 min and salting-out agent 1.5 g MgSO4. The linear range of the method was 1.0 mg/L-80 mg/L. The linear equation was as follows: Y=82670X+45930, r=0.9998. The detection limit of the method was 0.2 mg/L. The recovery rates ranged from 80.9% to 90.5%, and the precision was between 2.7% and 3.3%. Conclusions The sample pre-processing of the established method is simple, sensitive and accurate, and it can be applied to the rapid determination of methanol in plasma.
唐华民, 傅胜, 王安. 顶空-气相色谱-串联质谱法测定血浆中甲醇含量[J]. 实用预防医学, 2020, 27(3): 376-378.
TANG Hua-min, FU Sheng, WANG An. Determination of methanol in plasma by headspace gas chromatography-mass spectrometry. , 2020, 27(3): 376-378.
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2020, Vol. 27 
