Abstract：Objective To trace and detect the pathogen causing repeated diarrhea in a 9-month-old infant, and to provide evidence for the diagnosis of the illness. Methods According to the epidemiological survey, the infant’s stool, goat milk powder, rice flour and feeding bottles were sampled for the isolation and cultivation of common enteric pathogenic bacteria, and the isolated strains were subjected to pulsed field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Results After weaning, the infant drank goat milk as the staple food. Then he appeared repeated fever with diarrhea symptoms about half a month, and recovered after treatment with cefotaxime and vitamin C. Four strains of Enterobacter sakazakii were isolated from the stool sample, goat milk powder and two feeding bottles of the infant. The PFGE Results showed that 3 strains of Enterobacter sakazakii, including 1 strain isolated from goat milk powder and 2 strains from eluent samples of the 2 feeding bottles, shared the same PFGE pattern, which had 64.3% similarity with 1 strain isolated from the stool sample. Antimicrobial susceptibility testing indicated that the 4 strains of Enterobacter sakazakii were all sensitive to 10 antibiotics tested, including ampicillin, chloramphenicol, ciprofloxacin, azithromycin, imipenem, ceftazidime, gentamicin, nalidixic acid, compound sulfamethoxazole and tetracycline. Conclusions The pollution of Enterobacter sakazakii in infant formula goat milk powder should not be ignored, which may cause serious potential risk.
龙奇志, 何志敏, 陈友群, 林伟, 王岚, 胡世雄, 彭文伏, 贾华云. 一例由阪崎肠杆菌感染引起的婴儿腹泻调查[J]. 实用预防医学, 2017, 24(8): 954-956.
LONG Qi-zhi, HE Zhi-min, CHEN You-qun, LIN Wei, WANG Lan, HU Shi-xiong, PENG Wen-fu, JIA Hua-yun. One case of infant diarrhea induced by Enterobacter sakazakii infection. , 2017, 24(8): 954-956.
[1] Farmer JJ, Asbury MA, Hickman FW, et al. The enterobacteriaceae study group (USA). Enterobacter sakazakii : a new species of “Enterobacteriaceae” isolated from clinical specimens [J]. Int J Syst Bacteriol, 1980, 30:569-584. [2] Sheri K .Dennison , Joseph Morris .Multiresistant Enterobacter sakazakii wound infection in an adult[J] .Infections in Medicine, 2002, 19(11):533-535. [3] PulseNet. Standard Operating Procedure for PulseNet PFGE of Cronobacter Species [EB/OL].(2010-07-21)[2016-6-10]. https://www.cdc.gov/pulsenet/pdf/cronbacter-pfge-protocol-508c.pdf. [4] 范虹,李彦锋.国产微量肉汤稀释法药敏试剂检测常见多重耐药菌[J].国际检验医学杂志,2013,20(34):2735-2737. [5] 刘秀梅. 阪崎肠杆菌-食品安全控制的新目标[J]. 中国食品卫生杂志, 2004, 5(1):385-388. [6] 杨小蓉,黄伟峰,谢晓丽,等. 一例由阪崎肠杆菌感染引起腹泻患儿的溯源分析[J]. 疾病监测, 2014, 29(10):794-796. [7] 崔晶花,杨小蓉,杜小莉,等. 60株克罗诺杆菌的药敏分析[J]. 疾病监测,2012,27(5):409-411. [8] 张欣强,庞杏林,刘俊华,等.广州市售国产婴幼儿配方粉中阪崎肠杆菌污染调查[J].中国卫生检验杂志,2010,20(1):183-185. [9] 陈蓉,唐旭辉. 2011年永州市食源性致病菌监测[J].实用预防医学,2012,19(7):1032-1034. [10] 杨柳青,宋克云,苏良. 2010年长沙市食源性致病菌监测分析[J].实用预防医学,2011,18(5):859-860.
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