乙型肝炎病毒感染者血清HBV-LP检测意义及其与PreS1抗原、HBV DNA之间关系的研究

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摘要目的初步探讨 HBV 感染者血清中的乙肝大蛋白（HBV-LP）检测的临床意义,并对其与PreS1抗原、HBV DNA之间的关系进行分析。方法采用酶联免疫吸附试验 ( ELISA)方法检测 192 例 HBV 感染者血清中的HBV-LP, PreS1抗原及 HBV分子标志物（HBV M）,并用荧光定量 PCR 方法检测 HBV DNA。结果 192 例 HBV 感染者血清HBV-LP、PreS1抗原、HBV DNA及HBeAg阳性率分别为67.18%、54.17%、52.60%和9.38%,HBV-LP阳性率高于PreS1抗原阳性率（χ²=6.82,p<0.05）、HBV DNA检测阳性率（χ²=8.50,p<0.05）及HBeAg阳性率（χ²=135.80,p<0.05）;HBV-LP含量（A值）与HBV DNA 拷贝数的对数值呈正相关关系（r=0.798,P<0.05）。结论血清中 HBV-LP的含量与 HBV DNA 的拷贝数相关性较好,HBV-LP能准确的反应乙肝病毒复制情况,是HBeAg、PreS1抗原的重要补充。

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	程振波
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关键词 ：乙肝病毒包膜大蛋白, 乙肝病毒前S1抗原, 乙肝病毒DNA, 乙肝病毒标志物

Abstract：Objective To discuss the significance of hepatitis B virus large protein(HBV-LP) detection and the relationship among HBV-LP, PreS1 and HBV DNA copies. Methods Enzyme linked immuno-sorbent assay (ELISA) was used to detected the HBV-LP, PreS1-Ag, and HBV markers in 192 cases of infected serum,and the HBV DNA level was quantitatively measured by real-time polymerase chain reaction (PCR). Results In 192 cases of infected serum the positive rate of HBV-LP, PreS1-Ag,HBV DNA, and HBeAg were 67.18%、54.17%、52.60% and 9.38% respectively; the positive rate of HBV-LP was higher than that of PreS1-Ag（χ²=6.82,p<0.05）,HBV DNA（χ²=8.50,p<0.05）, and HBeAg（χ²=135.80,p<0.05）; there was a positive correlation between the concentration of HBV-LP(A value) and the HBV DNA level (r=0.798, P<0.05). Conclusion The levels of HBV-LP was positively related with the number of copies of HBV DNA . Serum HBV-LP was a new laboratory marker that can accurately reflect HBV DNA reproduction, and was a helpful complementarity to traditional HBeAg and PreS1-Ag.

Key words： Hepatitis B virus large protein Hepatitis B virus PreS1-Ag HBV DNA HBV markers

基金资助:湖南省医药卫生科研计划课题项目（C2013-065）

通讯作者: 谭黎明硕士生导师,湖南省人民医院检验科副主任,tanliming838@163.com

作者简介: 程振波检验师研究生湖南师范大学第一附属医院湖南长沙 410001 E-mail: zbcheng88hn@163.com

引用本文:

程振波, 谭黎明, 李建英, 毛玉环. 乙型肝炎病毒感染者血清HBV-LP检测意义及其与PreS1抗原、HBV DNA之间关系的研究[J]. 实用预防医学, 2016, 23(4): 487-489. CHENG Zhen-bo, TAN Li-ming, Li Jian-ying, et-al.. Significance of hepatitis B virus large protein (HBV-LP) detection and the relationship among HBV-LP, PreS1 and HBV DNA copies in infected serum. , 2016, 23(4): 487-489.

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https://www.syyfyx.com/CN/ 或 https://www.syyfyx.com/CN/Y2016/V23/I4/487

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