Abstract：Objective : to study the application of DNA ploidy analysis and Thinprep cytology test in early screening for cervical lesions. Methods : the retrospective analysis of 297 cases undergoing DNA ploidy analysis and Thinprep cytology test (TCT) from January to June in the year of 2014 in Hubei Maternal and Child Health Hospital, with biopsy under colposcope as gold standard. Results : 198 out of the 297 cases are DNA ploid negative. Among them, TCT Results were no intraepithelial lesion or malignancy (NILM) in 187 cases, ASCUS in 9 cases, and LSIL in 2 cases. Histological diagnosis by colposcopy biopsy were chronic cervicitis in 182 cases , CIN Ⅰin 11 cases ,CIN Ⅲ in 3 cases, cervical cancer in 2 cases. Among 99 cases of DNA ploid positive , TCT were NILM in 20 cases, ASCUS in 45 cases, LSIL in 23 cases, HSIL in 11 cases, while 37 cases were chronic cervicitis,25 cases were CINⅠ,9 cases were CIN Ⅱ, 18 cases were CIN Ⅲ, 10 cases were cancer confirmed by histological diagnosis. The sensitivity and negative predictive value of DNA ploidy analysis and Thinprep cytology test were 88.1%and 78.57%, 97.47% and 95.65% respectively, which shows no statistical differences. When both Methods were combined to predict CIN Ⅱor above pathological changes, the sensitivity and negative predictive value were 95.24％ and 98.93％respectively. Conclusion : DNA ploidy analysis can be applied in the early screening of cervical lesions. The combination of DNA ploidy analysis with Thinprep cytology test can lower the rate of missed diagnosis of cervical lesions due to high sensitivity.
阳艳, 段洁, 黄燕明, 郎雁, 王燕. DNA倍体分析和液基细胞学在宫颈病变早期筛查中的应用[J]. 实用预防医学, 2016, 23(4): 444-446.
Yang Yan, Duan Jie, Huang Yanming et al. Application of DNA ploidy analysis and Thinprep cytology test of cervical cells in early screening of cervix lesions. , 2016, 23(4): 444-446.
2016, Vol. 23 
