Abstract:Objective To investigate the contamination of food by Listeria monocytogenes and its changing trend in Shenyang,and to provide evidence for food safety risk assessment and early warning. Methods In accordance with the requirements of National Manual for Monitoring Listeria Monocytogenes, 509 food samples of 6 categories were randomly selected for monitoring of Listeria monocytogenes contamination. Results Twenty-six strains of Listeria monocytogenes were detected in the 509 samples, with the total detection rate of 5.1%. The detection rate varied significantly among various foods(χ2=16.72,P<0.01) , among seasons(χ2=18.46,P<0.01)and among foods from different sources(χ2=17.79,P<0.01), with the highest detection rates in the meat and meat products (11.5%), in the first (9.6%) and second seasons(9.4%) and in foods collected from farmers’ markets (13.8%). There was no significant difference in the detection rates of foods packaged in different ways(χ2=2.98,P>0.05). Conclusions There exists Listeria monocytogenes contamination in foods in Shenyang City in 2012-2014. Winter and spring are the high-occurrence seasons. Meat and meat products are high risk foods. Foods from farmers’ markets are more contaminated. Monitoring and management on key foods, key food consumption sites and relative diseases in key seasons should be strengthened to prevent and control food borne diseases.