Abstract：Objective To analyze the molecular biological characteristics of Vibrio parahemolyticus strains isolated from aquatic products and diarrhea patients in Fengtai District in 2013, to analyze the phylogenic relationship among the strains isolated from aquatic products and diarrhea patients, and to set up a molecular typing database for Vibrio parahemolyticus in Fengtai District. Methods Pulsed field gel electrophoresis (PFGE) was applied to molecular subtype of 72 Vibrio parahaemolyticus strains isolated from aquatic products and diarrhea patients, and BioNumerics version 5.10 software was used for clustering analysis of PFGE patterns. Results The PFGE showed that 50 strains from aquatic products could be divided into 48 patterns and 22 strains from diarrhea patients 13 patterns. Comparison of similarities of the strains revealed that 8 PFGE patterns were 100% homologous, while the similarities of other strains were all over 50%. Meanwhile, the similarity between the strains isolated from aquatic products and diarrhea patients was less than 80%. Conclusions There exist similarities in PFGE patterns of Vibrio parahemolyticus isolates from aquatic products and diarrhea patients in Fengtai District in 2013, but the correlation between them needs for further support from epidemiological evidence. The genotypes of Vibrio parahemolyticus strains from aquatic products exhibit polymorphism distribution, but no obvious predominant pulsotype of Vibrio parahemolyticus is found in the strains isolated from diarrhea patients.
[1] 陈晨,高永军,丁凡,等.2005-2012年我国其他感染性腹泻事件监测分析[J].实用预防医学,2014,21(6):695-697. [2] 李学云,石向辉,黄慈林,等.深圳市福田区2005-2007年感染性腹泻流行特征分析[J].实用预防医学,2009,16(1):88-90. [3] 安秀华,宁喜斌,李涛.水产品中副溶血性弧菌的污染、毒力基因及耐药性研究[J].食品科学, 2010,31(3):209-212. [4] 蒋立新,杨梅,邓凯杰,等.深圳市水产品中副溶血性弧菌污染现状及耐药性分析[J].职业与健康,2010,26(3)287-288. [5] 谭海芳,丁丽娜,林凤,等.肇庆市副溶血性弧菌血清分型和耐药性研究[J].实用预防医学,2010,17(2):349-352. [6] Yang ZQ, Jiao XA, Zhou XH, et al.Isolation and molecular characterization of Vibrio parahaemolyticus from fresh,low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China[J].Int J Food Microbiol,2008,125(3):279-285. [7] 赖晓华,肖新才,王鸣.珠江口水体及海、水产品致病性弧菌检测[J].中国公共卫生,2010,26(4):448-449. [8] 程苏云,翁景清,林香娟,等. 副溶血性弧菌食物中毒菌株的血清型、耐药性及基因检测[J].中国卫生检验杂志,2002,12(2):141-142. [9] 李晓春. 浙南部沿海地区细菌性食物中毒病原检测研究分析[J].中国预防医学杂志,2004,5(5):370-373. [10] 江艳华,姚琳,宋春丽,等.青岛市售贝类副溶血性弧菌污染状况及耐药性分析[J].中国卫生检验杂志,2012,22(2):375-377. [11] 兰进权,雷永良,柳付明,等.丽水市贝类中副溶血性弧菌的血清分型及耐药性[J].中国卫生检验杂志,2010,20(7):1763-1764. [12] 安秀华,宁喜斌.上海市市售水产品中副溶血性弧菌的分离、鉴定及耐药性研究[J].中国人兽共患病学报,2009,25(7):657-659. [13] 封会茹,曲梅,耿荣,等.2010-2012 年北京市丰台区感染性腹泻病原菌分布及耐药性分析 [J].疾病监测, 2013,28(2):96-100. [14] Tenover FC,Arbeit RD,Goering RV,et al. Interpreting chromosomal DNA restriction patterns produced by pulsed field gel electrophoresis: criteria for bacterial strain typing[J].J Clin Microbiol,1995,33(9):2233-2239. [15] Safa A,Bhuiyan NA,Alam M,et al. Genomic relatedness of the new matlab variants of Vibrio cholerae O1 to the classical and ElTor biotypes as determined by pulsed - field gel electrophoresis[J]. J Clin Microbiol,2005,43(3):1401-1404. [16] Centers for Disease Control and Prevention.Outbreaks of Escherichia coli O157:H7 infections among children associated with farm visits-Pennsylvania and Washington,2000 [J].MMWR Morb Mortal Wkly Rep,2001,50(15):293-297. [17] Gouveia S,Proctor ME,Lee MS,et al.Genomic comparisons and Shiga toxin production among Escherichia coli O157:H7 isolates from a day care center outbreak and sporadic cases in southeastern Wisconsin[J].J Clin Microbiol,1998,36(3):727-733. [18] 侯凤伶,刘维华,申志新,等.副溶血性弧菌脉冲场凝胶电泳分型研究[J].实用预防医学,2008,15(1):34-37. [19] 夏楚才,陈水波,苏华,等.副溶血性弧菌分子检测与分型研究进展[J].现代农业科技,2012,2(22):277-278. [20] 苗艳芳.脉冲场凝胶电泳用于副溶血性弧菌的分子流行病学研究[J].中国人兽共患病学报,2010,26(10):935-938.
2016, Vol. 23 
