Abstract：Objective To evaluate the specificity and sensitivity of the line probe assay in rapid detection of resistance to rifampin (RFP) and isoniazid (INH) in Mycobacterium tuberculosis in Benxi region. Methods Four hundred and fourteen sputum specimens from all registered smear-positive tuberculosis patients in the whole city were cultured by traditional Lwenstein-Jensen culture from March 2013 to June 2014. The positive cultures were examined by traditional drug sensitivity test, and the resistance to RFP and INH was determined by the line probe assay. The traditional drug sensitivity test as the golden standard was used to analyze the specificity and sensitivity of the line probe assay. Results Three hundred and ninety-four Mycobacterium tuberculosis strains were obtained. Compared with the traditional drug sensitivity test, the specificity and sensitivity of detecting resistance to RFP and INH and multi-drug resistance by the linear probe assay were 97.9% and 91.1%, 96.6% and 81.3%, 98.8% and 89.9% respectively. Conclusions The line probe assay is a rapid, sensitive, specific and effective method for the diagnoses of resistance to RFP and INH and multi-drug resistance in Mycobacterium tuberculosis. It can be popularized and applied in Benxi region.
邓闯,虞洋,周玲玲,赵庆成,靳志平. 线性探针技术快速诊断本溪地区耐多药肺结核效果评价[J]. 实用预防医学, 2016, 23(11): 1385-1387.
DENG Chuang, YU Yang, ZHOU Ling-ling, ZHAO Qing-cheng, JIN Zhi-ping. Evaluation on the line probe assay for rapid diagnosis of multidrug-resistant tuberculosis in Benxi region. , 2016, 23(11): 1385-1387.
2016, Vol. 23 
