Abstract：Objective To investigate the contamination status and pathogenic features of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province. Methods According to the aseptic sampling principle, 1,263 aquatic products of animal origin were collected from fair markets and supermarkets in 14 cities of Hunan Province. Vibrio parahaemolyticus was isolated and subjected to virulence gene detection, antimicrobial susceptibility testing and molecular typing by pulsed field gel electrophoresis (PFGE). Results Vibrio parahaemolyticus was detected in 221 of 1,263 aquatic products, with the detection rate of 17.50%. The trh gene was positive in 3 isolates and tdh gene in 1 isolate, with the virulence gene carrying rate of 1.81%. The isolates were the most resistant to ampicillin (30.32%), then to chloramphenicol (1.81%), sulfamethoxazole compound (1.36%) and tetracycline (0.45%). No multidrug resistance strain was detected. PFGE indicated that the bands of the isolates digested by Not I were relatively dispersed. Conclusions The contamination of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province is severe, and some isolates carry virulence gene and express resistance to antibiotics.
贾华云, 王岚, 陈帅, 张红, 梁进军, 刘晓革, 李俊华. 2010-2013年湖南省市售水产品中副溶血性弧菌污染状况及病原特征分析[J]. 实用预防医学, 2016, 23(12): 1433-1435.
JIA Hua-yun, WANG Lan, CHEN Shuai, ZHANG Hong, LIANG Jin-jun, LIU Xiao-ge, LI Jun-hua. Contamination status and pathogenic characteristics of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province, 2010-2013. , 2016, 23(12): 1433-1435.
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2016, Vol. 23 
