Abstract：Objective To explore the method of establishing the stable rabbit model of diabetes mellitus combined with syphilis, to observe a 14-week course of disease progression, to study the pathological damage of heart, lung, liver, kidney and testis and to preliminarily explore their causes. Methods The stable rabbit model of diabetes mellitus (group DM) was induced by alloxan. The stable rabbit model of syphilis (group Tp) was established by vaccinating Treponema pallidum in testis, and the stable rabbit model of diabetes mellitus combined with syphilis (group H) by injecting alloxan before vaccinating Treponema pallidum in testis. The healthy control group (group C) was also set up. The clinical manifestations of rabbits in each group, such as the changes of food intake, water intake, body weight, urine volume, hair color and mental state, were dynamically monitored. All the rabbits were sacrificed at the 14th week of the experiment. The heart, lung, liver, kidney and testis tissues were sectioned and stained with HE, and then the pathological damage of each tissue was observed and their causes were discussed preliminarily. Results The diabetes mellitus rabbit model, the syphilis rabbit model and the diabetes mellitus combined with syphilis model were successfully established. There appeared various degrees of changes in food intake, water intake, body weight, urine volume, hair color and mental state in the rabbits of group DM, group Tp and group H. Compared with the healthy control group, the organs of group DM, group Tp and group H showed pathological changes at the 14th week of the experiment, including edema, inflammation and other pathological changes in heart tissue, inflammatory cell infiltration in lung tissue, cellular swelling, inflammation, even nodular necrosis and other changes in liver tissue, edema, vasodilation, inflammation, significant tubular protein and calcium deposi-tion in kidney tissue, and stromal cell hyperplasia, multiple inflammatory cell infiltration and nodular necrosis in testicular tissue. Group H showed more obvious pathological changes as compared with group DM and group Tp. Conclusions Compared with diabetes mellitus rabbit model and syphilis rabbit model, the rabbit model of diabetes mellitus combined with syphilis displays more severe pathological damages in heart, lung, liver, kidney and testis. It may be related to a severe inflammatory response to oxidative stress accelerated by diabetes mellitus combined with syphilis infection.
刘炀, 刘双全, 谭浩, 罗韬. 糖尿病合并梅毒兔模型的建立及其组织病理损伤的研究[J]. 实用预防医学, 2017, 24(9): 1025-1031.
LIU Yang, LIU Shuang-quan, TAN Hao, LUO Tao. Establishment of diabetes mellitus combined with syphilis model in rabbits and research on ita histopathological damage. , 2017, 24(9): 1025-1031.
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