Correlation of serum IL-1β and IL-8 expression levels with serum reagin titer in patients with syphilis
DENG Yi1, LEI Ming1, WU Xue-feng1, ZHAO Fei-jun2
1. The First People's Hospital of Changde City, Changde, Hunan 415003, China; 2. Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, China
Abstract：Objective To explore the correlation between the expression levels of serum interleukin-1β (IL-1β) andinterleukin-8 (IL-8) and serum reagin titer in patients with syphilis at different clinical stages. Methods Serum samples were collected from 40 physical examinees and 120 patients with syphilis (who were divided into the primary syphilis group, the secondary syphilis group and the latent syphilis group). The serum levels of IL-1β and IL-8 in each group were measured by enzyme-linked immunosorbent assay. The toluidine red unheated serum test (TRUST) was used to test serum reagin titers of the syphilis patients. According to the TRUST titers, the syphilis patients were separated into the high titer group and the low titer group, and the differences in the expression levels of IL-1β and IL-8 between the groups were compared. Results The expression levels of serum IL-1β and IL-8 were significantly different among the groups (P<0.05), of which serum IL-1β (15.10 (10.63,19.16)) pg/ml and IL-8 (108.67 (69.96, 139.79)) pg/ml were significantly higher in the secondary syphilis group than in the primary syphilis group, the latent syphilis group and the normal group. Serum IL-8 (86.03(62.25,144.94)) pg/ml was significantly higher in the high titer group than in the low titer group (57.21(52.16, 86.13)) pg/ml, and the difference was statistically significant (P<0.05). However, no statistically significant difference was found in IL-1β between the high titer group (11.12 (7.18, 15.96) pg/ml) and the low titer group (8.43 (6.93, 14.05) pg/ml) (P>0.05). There was a positive correlation between serum IL-8 level and reagin titer in patients with secondary syphilis (r=0.625, P<0.05). Conclusion Serum IL-1β and IL-8 were expressed at high levels in patients with primary, secondary and latent syphilis, and there was a certain correlation between changes in serum levels of IL-1β and IL-8 expression and the disease progression of syphilis.
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