Abstract:Objective To analyze nasopharyngeal carriage of Streptococcus pneumoniae serotypes and characteristics of drug resistance in healthy children under 6 months of age in Jinan, and to provide reliable data for pneumococcal vaccination. Methods Two hundred and sixteen nasopharyngeal swabs were collected from healthy infants younger than 6 months in Jinan from November 2019 to April 2020. Streptococcus pneumoniae was cultured and isolated. Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and optochin susceptibility were performed. Multiplex PCR was used to determine Streptococcus pneumoniae serotypes. Antibiotic susceptibility was evaluated by antimicrobial susceptibility analyzer. Results The carriage rate of Streptococcus pneumoniae in the 216 healthy children under 6 months of age was 5.1% (11/216), including seven pneumococcal serotypes, 15B, 23F, 6B, 8, 18C, 19F and 13. The vaccine coverage of the 13-valent pneumococcal polysaccharide conjugate vaccine was 54.5%, and that of the 23-valent pneumococcal polysaccharide vaccine was 90.9%. Among 19 antibiotics, the drug resistance rates of erythromycin and azithromycin were 100.0%, those of tetracycline, clindamycin and trimethoprim/sulfamethoxazole were over 80.0%, and those of cefuroxime and penicillin (oral) were over 50.0%. No drug resistant strains were found for vancomycin, linezolid, levofloxacin, chloramphenicol and moxifloxacin. Streptococcus pneumoniae isolated from eleven children’s nasopharynx showed multi-drug resistance with 3-6 kinds of drugs. ConclusionStreptococcus pneumoniae colonized in nasopharynx of the healthy children under 6 months of age in Jinan has multiple serotypes and serious antimicrobial resistance.
关恒云, 刘岚铮, 姜慧钰, 白爱英, 刘辉, 阮师漫. 健康6月龄内儿童肺炎链球菌携带状况及耐药特征分析[J]. 实用预防医学, 2023, 30(2): 169-172.
GUAN Heng-yun, LIU Lan-zheng, JIANG Hui-yu, BAI Ai-ying, LIU Hui, RUAN Shi-man. Carriage of Streptococcus pneumoniae and its drug resistance characteristics in healthy children under 6 months of age. , 2023, 30(2): 169-172.
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