2021年长沙市食源性疾病沙门菌血清分型及PFGE分子分型分析

doi:10.3969/j.issn.1006-3110.2022.09.002

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摘要目的了解长沙市食源性疾病沙门菌的血清分型和PFGE分子分型情况,为食源性疾病防控提供依据。方法按《食源性疾病监测工作手册》收集长沙市4所哨点医院食源性疾病病例粪便标本,从中分离沙门菌,并对沙门菌进行血清分型和凝胶电泳(pulsed field gel electrophoresis,PFGE),通过Bionumerics 7.6 软件对电泳图谱进行分析。结果全年共监测528份标本,检出沙门菌50株,检出率为9.47%,阳性病例主要集中在5岁及以下年龄段,占74.00%(37/50),发病主要在夏秋5—9月,占全年的76.00%(38/50)。血清型共有12种,主要血清型是鼠伤寒沙门菌占60.00%(30/50),其次为斯坦利沙门菌4株占8.00%,肠炎沙门菌3株占6.00%。 50株沙门菌PFGE分型为41种带型,相似度为55.7％～100％,呈现多态性,主要分成两个簇,有8组相似度 100％的菌株,共17株(占34.00%,17/50),主要是鼠伤寒沙门菌;有10簇相似度90%以上的克隆系,包含32株沙门菌。结论长沙市2021年食源性疾病沙门菌感染的优势血清型是鼠伤寒沙门菌,沙门菌PFGE分子分型带型呈多态化,PFGE分型可识别共同感染来源的病例,可用于暴发疫情识别和控制。

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	苏良
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	杨柳青
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	张玉娟
	曹可珂

关键词 ：食源性疾病, 沙门菌, 脉冲场凝胶电泳

Abstract：Objective To understand the serotyping and PFGE molecular typing of Salmonella isolates from foodborne diseases in Changsha City, and to provide a basis for prevention and control of foodborne diseases. Methods Faecal samples of foodborne disease cases in 4 sentinel hospitals in Changsha City were collected according to the Guidelines for Active Surveillance of Foodborne Diseases. Salmonella was isolated from the samples, and serological typing and pulsed field gel electrophoresis (PFGE) of Salmonella were performed. The electrophoretic map was analyzed by Bionumerics 7.6 software. Results A total of 528 samples were monitored throughout the year, and 50 strains of Salmonella were detected, with the detection rate of 9.47%. The positive cases were mainly in the age group of 5 years old and below, with the proportion being 74.00% (37/50). The incidence was mainly from May to September in summer and autumn, accounting for 76.00% (38/50) of the whole year. 12 serotypes were identified, and the main serotype was Salmonella typhimurium (60.00%, 30/50), followed by Salmonella Stanley (8.00%, 4/50) and Salmonella enteritis (6.00%, 3/50). PFGE typing of 50 strains of Salmonella was divided into 41 PFGE bands with similarity value of 55.7%-100%, showing polymorphism, and they were mainly divided into two clusters. There were 8 groups of strains with similarity of 100%, and a total of 17 strains with composition ratio of 34.00% (17/50). They were mainly Salmonella typhimurium. There were 10 clonal lines containing 32 strains of Salmonella with similarity of more than 90%. Conclusion The dominant serotype of Salmonella infection in foodborne diseases in Changsha City in 2021 was Salmonella typhimurium. PFGE molecular typing was polymorphic. PFGE typing can identify cases of common infection source and can be used for outbreak identification and control.

Key words： foodborne disease Salmonella pulsed field gel electrophoresis

收稿日期: 2022-02-09

R155.3

作者简介: 苏良(1979-),男,本科,副主任技师,研究方向:细菌性疾病检测和防控。

引用本文:

苏良, 宋迎春, 杨柳青, 刘晓雷, 张玉娟, 曹可珂. 2021年长沙市食源性疾病沙门菌血清分型及PFGE分子分型分析[J]. 实用预防医学, 2022, 29(9): 1028-1030. SU Liang, SONG Ying-chun, YANG Liu-qing, LIU Xiao-lei, ZHANG Yu-juan, CAO Ke-ke. Serotypes and PFGE molecular typing of Salmonella isolated from foodborne diseases in Changsha City, 2021. , 2022, 29(9): 1028-1030.

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https://www.syyfyx.com/CN/10.3969/j.issn.1006-3110.2022.09.002 或 https://www.syyfyx.com/CN/Y2022/V29/I9/1028

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