Abstract：Objective To study the etiology of patients with polycystic ovary syndrome (PCOS) by using tandem mass tag (TMT) combined with liquid chromatography/tandem mass spectrometry (LC-MS/MS), differentially expressed proteins and associated biological pathways. Methods Twenty patients with PCOS were enrolled into this study to serve as the PCOS group, and 18 healthy women were considered as the control group. The statistical and bioinformatics data were analyzed using all fasting serum samples detected by TMT and LC-MS/MS. Results Thirty-eight differential expression proteins were identified with 21 up-regulated expression (FC≥1.2, P<0.05) and 17 down-regulated expression (FC≤0.83, P<0.05) in PCOS. These proteins were enriched using Kyoto Encyclopedia of Genes and Genomes (KEGG) and Reactome to 18 and 78 pathways, respectively. Integrating two databases enrichment analyses found the enrichment pathway of complement cascade reaction, and the differential expression protein common involved in these databases was mannose-binding protein (MBL). Conclusion PCOS patients may have overactivation in the complement system in which the differential protein MBL may be a key pathogenic factor.
邹颖, 马丹, 穆仪冰, 方超英. 血清TMT标记定量蛋白质组学对多囊卵巢综合征病因研究[J]. 实用预防医学, 2021, 28(11): 1311-1314.
ZOU Ying, MA Dan, MU Yi-bing, FANG Chao-ying. Study on the etiology of polycystic ovary syndrome using serum tandem mass tag-based quantitative proteomics. , 2021, 28(11): 1311-1314.
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