多囊卵巢综合征患者血清HSP70、sICAM-1与胰岛素抵抗及卵巢功能的关系研究

doi:10.3969/j.issn.1006-3110.2021.09.014

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摘要目的观察多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者的血清热休克蛋白70(Heat shock protein 70,HSP70)、可溶性细胞间粘附分子-1(soluble intercellular adhesion molecules-1,sICAM-1,sICAM-1)的表达情况及其与胰岛素抵抗(homeostasis model assessment of insulin resistance, HOMA-IR)及卵巢功能的关系。方法选取2019年2月—2020年8月在东营市第五人民医院接受治疗的多囊卵巢综合征患者40例作为观察组,选取同期在该院接受体检的40例健康成年女性为对照。酶联免疫吸附法检测血清HSP70、sICAM-1水平,并检测两组患者血糖、胰岛素及性激素水平,比较两组患者空腹血糖、胰岛素、性激素、HSP70、sICAM-1水平差异,并分析HSP70、sICAM-1与HOMA-IR及性激素水平间的相关关系。结果观察组患者的HSP70、sICAM-1水平[(0.29±0.06)ng/ml、(425.42±15.38)ng/ml]高于对照组[(0.16±0.05)ng/ml、(158.35±10.15)ng/ml] (P<0.05),观察组空腹血糖(Fasting blood glucose, FBG)、空腹胰岛素(Fasting insulin, Fins)及HOMA-IR[(5.96±1.01)mmol/L、(11.09±2.03)mIU/L、(2.78±0.36)]高于对照组[(4.24±0.95)mmol/L、(9.74±1.17)mIU/L、(2.09±0.42)] (P<0.05),观察组卵泡刺激素(Follicle stimulating hormone, FSH)、雌二醇(Estradiol, E2 )水平[(3.98±1.03)pmol/L、(40.23±4.17)pmol/L]均低于对照组[(5.62±1.14)pmol/L、(52.38±5.43)pmol/L](P<0.05),黄体生成素(Luteinizing hormone, LH)及睾酮(Testosterone, T)水平[(7.85±1.11)pmol/L、(3.62±0.44)nmol/L]高于对照组[(4.76±1.05)pmol/L、(2.19±0.26)nmol/L] (P<0.05)。相关分析结果显示,PCOS 患者血清HSP70及sICAM-1与HOMA-IR均呈现正相关关系(P<0.05),与血清LH、T水平均呈现正相关关系,与血清FSH及E2水平呈现负相关关系。结论多囊卵巢综合征患者血清HSP70、sICAM-1水平升高,其可能通过调节胰岛素抵抗状态参与对卵巢功能的影响。

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关键词 ：多囊卵巢综合征, 热休克蛋白, 胰岛素抵抗, 卵巢功能

Abstract：Objective To observe the expression of serum heat shock protein 70 (HSP 70) andsoluble intercellular adhesion molecules-1 (sICAM-1) and its relationship with homeostasis model assessment of insulin resistance (HOMA-IR) and ovarian function in patients with polycystic ovary syndrome (PCOS). Methods Forty PCOS patients treated in Dongying Fifth People’s Hospital from February 2019 to August 2020 were selected as the observation group, while 40 healthy women undergoing physical examination in this hospital were simultaneously selected as the control group. ELISA was used to detect the serum levels of HSP70 and sICAM-1. Blood sugar, insulin and sex hormone levels were also measured. Differences in the levels of fasting blood glucose (FBG), insulin, sex hormone, HSP70 and sICAM-1 between the two groups were compared. The correlation of HSP70 and sICAM-1 with HOMA-IR and sex hormone was analyzed. Results Levels of HSP70 and sICAM-1 were higher in the observation group ((0.29±0.06) ng/ml, (425.42±15.38) ng/ml) than in the control group ((0.16±0.05) ng/ml, (158.35±10.15) ng/ml) (P<0.05). FBG, fasting insulin and HOMA-IR were higher in the observation group ((5.96±1.01) mmol/L, (11.09±2.03) mIU/L, (2.78±0.36)) than in the control group ((4.24±0.95) mmol/L, (9.74±1.17) mIU/L, (2.09±0.42)) (P< 0.05). Levels of follicle stimulating hormone (FSH) and estradiol (E2) were lower in the observation group ((3.98±1.03)pmol/L, (40.23±4.17) pmol/L) than in the control group ((5.62±1.14) pmol/L, (52.38±5.43) pmol/L) (P<0.05). Levels of luteinizing hormone (LH) and testosterone (T) were higher in the observation group ((7.85±1.11) pmol/L, (3.62±0.44) nmol/L) than in the control group ((4.76±1.05) pmol/L, (2.19±0.26) nmol/L)(P<0.05).The correlation analysis revealed that serum levels of HSP70 and sICAM-1 in the PCOS patients were positively correlated with HOMA-IR, LH and T inserum(P<0.05), but negatively correlated with levels of FSH and E2 in serum. Conclusion Serum levels of HSP70 and sICAM-1 in the PCOS patients are elevated, which may contribute to the influence of ovarian function by regulating insulin resistance.

Key words： polycystic ovary syndrome heat shock protein insulin resistance ovarian function

收稿日期: 2021-05-03

R711.75

作者简介: 张宗峰(1973-),男,大学本科,主管技师,研究方向:医学检验。

引用本文:

张宗峰, 刘兴云, 李玉军. 多囊卵巢综合征患者血清HSP70、sICAM-1与胰岛素抵抗及卵巢功能的关系研究[J]. 实用预防医学, 2021, 28(9): 1084-1086. ZHANG Zong-feng, LIU Xing-yun, LI Yu-jun. Relationship of serum HSP70 and sICAM-1 with insulin resistance andovarian function in patients with polycystic ovary syndrome. , 2021, 28(9): 1084-1086.

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https://www.syyfyx.com/CN/10.3969/j.issn.1006-3110.2021.09.014 或 https://www.syyfyx.com/CN/Y2021/V28/I9/1084

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