Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identification of rare Ochrobactrumin children with bloodstream infection and analysis of the drug-resistance
LIU Jian-long, GUO Kuan-peng, LI Xian-bin, SONG Chun-rong, HU Qiong, TANG Xi-chun
Center of Clinical Laboratory Medicine, Hunan Children’s Hospital, Changsha, Hunan 410007, China
Abstract：Objective To rapidly identify and analyze rare Ochrobactrum and its drug-resistance situation in children with bloodstream infection. Methods We collected 26 strains of Ochrobactrum isolated from clinical specimens from children with bloodstream infection in a children’s hospital. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to detected the strains, and then they were identified based on biochemical phenotype and gene sequencing analysis. According to the results of drug susceptibility test, the drug-resistance genes were analyzed by PCR method. Results Among the 26 strains detected by MALDI-TOF MS and 16S rRNA gene sequencing, 25 strains were identified as Pseudochrobactrum saccharolyticum. Drug sensitivity results showed that their resistance rates to piperacillin/tazobactam, quinolones, aminoglycosides and cotrimoxazole were 0, but their resistance rate to amtriaxone was 100%. The other β-lactam antibacterial drugs had different drug-resistance rates. AmpC and AmpR gene tests were negative. Conclusion MALDI-TOF MS can rapidly identify clinical rare Ochrobactrum. The strains of Pseudochrobactrum saccharolyticum isolated from children with bloodstream infection do not contain AmpC or AmpR resistance genes. It is recommended to use piperacillin/tazobactam based onthe results of drug sensitivity analysis combined with the characteristics of medication in children.
刘健龙, 郭宽鹏, 李先斌, 宋春荣, 胡琼, 唐喜春. 基质辅助激光解吸电离飞行时间质谱在鉴定儿童血流感染少见苍白杆菌的应用及耐药情况分析[J]. 实用预防医学, 2021, 28(7): 818-821.
LIU Jian-long, GUO Kuan-peng, LI Xian-bin, SONG Chun-rong, HU Qiong, TANG Xi-chun. Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identification of rare Ochrobactrumin children with bloodstream infection and analysis of the drug-resistance. , 2021, 28(7): 818-821.
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2021, Vol. 28 
