Effect of restricted feeding on serum beta-hydroxybutyrate level and lipoprotein lipase expression in cerebral cortex in mice
LI Xin-hui*, ZHAN Zhi-peng, ZHANG Jing-zhu, REN Ya-hao, ZHAO Yue, AN Li
*Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, Shenyang, Liaoning 110122, China Corresponding author: AN Li, E-mail:anli@ cmu.edu.cn
Abstract：Objective To explore the effect of restricted feeding on the serum beta-hydroxybutyrate (βOHB) level and lipoprotein lipase (LPL) expression in cerebral cortex in mice. Methods Based on their body weight, 50 healthy adult C57BL/6J mice were randomly divided into 5 groups (each n=10), and blood was respectively collected at 0, 8, 12, 16 and 24 h after fasting for detection of serum βOHB by ELISA. Additionally, the other 20 mice were randomly divided into 2 groups (each n=10), the fasting group and control group. The mice in the fasting group received alternate-day fasting diet for 4 consecutive weeks, while those in the control group were fed ad libitum. The body weight and the percentage of body fat were measured at the end of the experiment. Moreover, the expression of LPL mRNA and protein in the cerebral cortex of mice were detected by real-time RT-PCR and Western blot respectively. Results The serum βOHB level in mice was gradually increased with the extension of fasting time, with (0.93±0.13) nmol/L, (0.95 ± 0.37) nmol/L, (1.33 ± 0.26) nmol/L, (1.68 ± 0.13) nmol/L and (1.74 ± 0.27) nmol/L at 0, 8, 12, 16 and 24 h after fasting respectively. Compared with the serum βOHB level at 0 h, the βOHB levels at 12, 16 and 24 h after fasting were significantly increased (all P<0.05). No significant difference was found between the serum βOHB levels at 16 and 24 h after fasting (P>0.05), but both were remarkably higher than that at 12 h (P<0.05). During the experiment, no abnormal manifestations were found in the mice of the alternate-day fasting group and the control group; moreover, their body weight and percentage of body fat showed no statistically significant difference (P>0.05). Compared with the control group, the expression of LPL mRNA and protein in the cerebral cortex of the mice in the alternate-day fasting group was remarkably up-regulated(P＜0.01; P＜0.05). Conclusions The serum βOHB level in mice is significantly increased during 12-24 h after fasting, and alternate-day fasting for 4 weeks can up-regulate the expression of LPL mRNA and protein in the cerebral cortex of mice.
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2017, Vol. 24 
