Abstract：Objective To develop an analytical method based on high performance liquid chromatography (HPLC) for determination of 7 kinds of functional compositions (including L-carnitine, L-theanine, catechin, chromium picolinate, sennoside A, sennoside B and carnosic acid) in slimming health food. Methods Samples were extracted by 50% acetonitrile. The chromatographic separation was performed on C18 column using a mobile phase consisting of methanol and potassium dihydrogen phosphate solution (0.1% phosphoric acid) under gradient mode. And external standard method was used for quantitative analysis.Results A good linearity was got in the range of 10.0 mg/L-100.0 mg/L for L-carnitine, L-theanine and carnosic acid, in the range of 1.0 mg/L-50.0 mg/L for catechin, in the range of 0.50 mg/L-10.0 mg/L for sennoside A, in the range of 0.20 mg/L-10.0 mg/L for sennoside B, and in the range of 2.0 mg/L-50.0 mg/L for chromium picolinate. The correlation coefficients were equal to or greater than 0.9991. The recoveries were in the range of 89.9%-108.0%, the relative standard deviation was between 1.2% and 6.3%, and the limits of detection were 0.1 mg/kg-5 mg/kg. Conclusions This method is rapid, accurate and feasible, and it is suitable for simultaneous quantification of 7 kinds of functional compositions in slimming health food.
2020, Vol. 27 
