Abstract:Objective To study the genotype distribution and drug resistance of newly-confirmed HIV-1 positive cases without antiretroviral therapy (ART) through different pathways of infection in Zhuhai, Guangdong province in 2018. Methods Two hundred and seven plasma samples were collected from the research subjects. Viral RNA was extracted, and HIV-1 pol gene was amplified by nested RT-PCR and sequenced. According to pol gene sequences, the subtype distribution and drug resistance of HIV-1 were analyzed. Results The epidemic genotypes in MSM were CRF07_BC(43.3%), CRF01_AE(36.1%)and CRF55_01B(13.4%), and those in heterosexually-acquired HIV infection cases were CRF01_AE(52.5%), CRF07_BC(31.1%) and CRF08_BC(6.6%). CRF01_AE was detected in three injecting drug users, and H subtype in one case of blood-borne infection. HIV-1 infection in MSM and heterosexually-acquired HIV infection cases showed low drug resistance, with the low drug resistance rates of 4.1% and 4.9%, respectively. No drug resistance case was detected in the injecting drug users and theblood-borne infection case. Conclusions CRF01_AE and CRF07_BC were predominant in sexually transmitted HIV-1 infections, and many other genotypes were prevalent together.Intermediate and high-level resistancecases were not detected in the above-mentioned four kinds of populations. Transmissible drug resistance rate was generally low in Zhuhai.