Significance of SP-D and SP-A expression in alveolar lavage fluid and serum of patients with coal worker’s pneumoconiosis and their correlation with pulmonary inflammation
HU Zhuo-jun, LYU Lin, SHEN Chun-ying, PANG Bo, DONG Ming-xia, LIU Li, LIU Jian-hong
Department of Occupational Diseases, the Second People’s Hospital of Panzhihua City, Panzhihua, Sichuan 617068, China
Abstract：Objective To study the significance of alveolar surfactant protein D (SP-D) and alveolar surfactant protein A (SP-A) expression in alveolar lavage fluid and serum of patients with coal worker’s pneumoconiosis (CWP) and their correlation with pulmonary inflammation. Methods Eighty-eight CWP patients admitted from August 2016 to June 2019 were selected as the case group, and 92 non-CWP underground workers with the same dust contact condition in the same mining area severed as the control group. The levels of SP-D and SP-A in serum and alveolar lavage fluid were compared between the two groups, and their diagnostic values for CWP were analyzed. At the same time, we compared the levels of SP-D, SP-A, and inflammatory factors (including interferon gamma (IFN-γ) and interleukin-10 (IL-10)) in serum and alveolar lavage fluid of patients in different stages and correlation of SP-D and SP-A with IFN-γ and IL-10. Results The levels of SP-D ((46.24±10.44)μg/ml, (40.26±13.40)μg/ml) and SP-A ((42.17±7.30)μg/ml, (11.28±3.74)μg/ml) in serum and alveolar lavage fluid in the case group were all lower than those in the control group ( the levels of SP-D were (57.63±13.69)μg/ml and (94.18±31.35)μg/ml, repectively; the levels of SP-D were (49.55±9.12)μg/ml and (63.75±21.20)μg/ml, respectively) (all P<0.05). Analysis of receiver operating characteristic (ROC) curve and area under the curve (AUC) showed combined use of SP-D and SP-A in alveolar lavage fluid for the diagnosis (0.901) > SP-D in alveolar lavage fluid (0.873) > SP-A in alveolar lavage fluid (0.863) > SP-D in serum (0.788) > SP-A in serum (0.764). SP-D and SP-A in serum and alveolar lavage fluid were negatively correlated with IFN-γ and IL-10 (r1=-0.528, r2=-0.410, r3=-0.549, r4=-0.544, all P<0.05). Conclusions SP-D and SP-A are under-expressed in alveolar lavage fluid and serum of CWP patients, and have a negative correlation with pulmonary inflammation. Combined use of SP-D and SP-A in alveolar lavage fluid is expected to become an important and effective biomarker for clinical differential diagnosis, judgment of disease stage, and assessment of pulmonary inflammation.
胡卓君, 吕林, 沈春鹦, 庞波, 董明霞, 刘丽, 刘建红. SP-D、SP-A在煤工尘肺病患者肺泡灌洗液、血清中的表达意义及与肺部炎症相关性[J]. 实用预防医学, 2020, 27(11): 1349-1352.
HU Zhuo-jun, LYU Lin, SHEN Chun-ying, PANG Bo, DONG Ming-xia, LIU Li, LIU Jian-hong. Significance of SP-D and SP-A expression in alveolar lavage fluid and serum of patients with coal worker’s pneumoconiosis and their correlation with pulmonary inflammation. , 2020, 27(11): 1349-1352.
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2020, Vol. 27 
