Abstract：Objective To establish a method for the simultaneous determination of common organic phosphorus pesticide residues in vegetables by solid phase extraction coupled with gas chromatography (SPE- GC). Methods Samples were extracted by acetonitrile, cleaned up on a solid phase column purification and determined by SPE-GC. Results Under the selected conditions, up to 19 kinds of organic phosphorus pesticide could be completely separated and showed good linearity. The correlation coefficient (r) was ≥ 0.9994, with the detection limit of 2.5-16.9 μg/kg. The recovery rate of standard addition was 62.4%-111.5%, and the relative standard deviation (RSD) was 0.74%-5.26%. Conclusions The experimental results show that the method is simple, highly sensitive and accurate, with a good reproducibility. It is suitable for the determination and analysis of the common organophosphorus pesticide residues in vegetables.
[1] 张敬平, 刘文卫, 周闰, 等. 气相色谱法同时检测生活饮用水中的27种有机磷农药残留[J]. 中国卫生检验杂志, 2010, 20(6):527-528. [2] 朱坚, 王国权, 陈正夫, 等.食品中危害残留物的现代分析技术[M].上海:同济大学出版社,2003:101. [3] 杨龙江, 刘拉平, 李岚, 等. 蔬菜中有机磷农药残留研究与对策[J]. 环境污染与防治, 2003, 25(6):370-371. [4] Undeger U, Basaran N. Effects of pesticedes on human peripheral lymphocytes in vitro: induction of DNA damage[J]. Arch Toxicol, 2005, 79:169-176. [5] Rahman MF, Mahboob M, Danadevi K, et al. Assessment of genotoxic effects of chloropyriphos andacephate by the comet assay in mice leucocytes[J]. Mutat Res, 2002,516:139-147. [6] Padungtod C, Hassold TJ, Elise Millie, et al. Spermaneupioidy among Chinese pesticide factory workers:Scoring by the FISH method [J]. Am J Ind Med, 1999, 36(2):230-238. [7] Zahran MM, Abdel AK, Abdel RA, et al. The effect of subacute doses of organophosphorus pesticide, nuvacron, on the biochemical and cytogenetic parameters of mice and their embryos[J]. Res J Agr Biol Sci, 2005, 1(3):277-283. [8] 于鸿, 陈安, 李晓晶, 等.固相萃取结合气相色谱法测定蔬菜中33种有机磷农药的残留量[J].中国食品卫生杂志治, 2011, 23(4):330-335. [9] 易承学, 徐虹, 何凯, 等. 金针菇中有机磷农药残留的快速检测方法研究[J]. 实用预防医学, 2015, 22(5):621-623. [10] 王海凤, 王俊斌, 刘海学, 等. 气质联用法同时测定果蔬中多种有机磷农药残留[J]. 食品与机械, 2013, 29(1):92-94. [11] JI J, Deng CH, Zhang HQ, et al.Wicrowave assisted steam distillation for the determination of organochlorine pesticides and pyrethroids in Chinese teas [J]. Talanta, 2007, 71:1068 -1074. [12] 廖和菁, 张雪春, 胡礼渊, 等. 气相色谱法同时测定茶叶中10种有机磷农药残留[J]. 中国食品卫生杂志, 2015, 27(1):41-44. [13] 黄芬, 夏文斌, 李雄伟, 等. 气相色谱法同时测定水中19种常见有机磷农药残留[J]. 健康世界, 2015, 23(1):61-62. [14] 中华人民共和国国家质量监督检验检疫总局. GB/T 8855-2008新鲜水果和蔬菜取样方法[S].北京:中国标准出版社,2008:1-2. [15] 中华人民共和国农业部. NY/T 761-2008蔬菜和水果中有机磷、有机氯、拟除虫菊酯和氨基甲酸酯类农药多残留测量法[S].北京:中国标准出版社,2008:1-9.
2017, Vol. 24 
