Abstract：Objective To study the toxicological safety of extractive from Anoectochilus roxburghii leaves. Methods According to the Food Safety Toxicology Evaluation Procedures and Methods, we conducted the tests like acute oral toxicity test in mice, genetic toxicity test in mice (Ames test, bone marrow cell micronucleus test and sperm abnormality test), teratogenic test and 90-day feeding test in rats. Results The maximum tolerated dose of mice treated with Anoectochilus roxburghii leaves was more than 35.00 g/(kg·bw), which belonged to an actual non-toxicity substance. The result of Ames test was negative under 8-5,000 μg/plate dosage. No positive results were found in micronuclear test and sperm abnormality test under 2.50-10.0 g/(kg·bw)dosage of extractive fromAnoectochilus roxburghii leaves. There was no toxicity in pregnant rats and embryo, and no teratogenicity was found in rat embryo treated with 2.92-8.75 g/(kg·bw) dosage of extractive from Anoectochilus roxburghii leaves. There were no related adverse effects on body weight, food intake, food consumption, hematology, serum biochemistry and histopathology in rats under 5.83-17.50 g/kg·bwdosage of extractive from Anoectochilus roxburghii leaves. Conclusions Under these experimental conditions, no obvious toxic and side effects were observed in extractive from Anoectochilus roxburghii leaves.
刘志光, 胡余明, 尹晓晨, 易传祝, 何珊, 张莹莹, 李梓民. 金线莲植物叶提取物毒理学安全性评价的实验研究[J]. 实用预防医学, 2020, 27(9): 1072-1076.
LIU Zhi-guang, HU Yu-ming, YIN Xiao-chen, YI Chuan-zhu, HE Shan, ZHANG Ying-ying, LI Zhi-min. Experimental study on toxicological safety evaluation of extractive from Anoectochilus roxburghii leaves. , 2020, 27(9): 1072-1076.
2020, Vol. 27 
