Test results for detection of antibody against Lyme disease in certain patients in the northeast of Hainan province
LI Sha1, ZHANG Lin2, LI Huan1, HOU Xue-xia2, CHEN Ting1, MIAO Guang-qing2, FU Xiao-ying1, CHEN Hai1, HAO Qin2, ZHU Xiong1
1. The People's Hospital of Sanya City, Sanya, Hainan 572000, China; 2. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract：Objective To investigate the prevalence of Lyme disease among people in the northeast of Hainan province so as to provide references for clinicians' diagnosis and a reliable theoretical basis for epidemiological investigation of Lyme disease in Hainan province. Methods We collected 1,334 serum samples from patients with arthritis and/or neurological symptoms in three hospitals in the northeast of Hainan province. Firstly, indirect fluorescent antibody (IFA) test was used to detect serum antibodiesagainst Lyme disease, and secondly, the positive samples detected by IFA test were confirmed by Western blot (WB) test. Results Among the 1,334 patients detected by IFA test in the northeast of Hainan province, 60 cases were positive for antibodies against Lyme disease, and the positive rate was 4.50%. The positive rates of Lyme disease antibodies detected by IFA in the 1,334 patients from the three hospitals in Haikou, Wenchang and Qionghai were 4.25%, 1.40% and 8.28%, respectively, showing statistically significant differences among the three hospitals (χ2=25.640, P<0.05). No statistically significant differences were found in the positive rates of antibodies to Lyme disease between different genders and among age groups (χ2=2.306, P>0.05; χ2=1.015, P>0.05). WB test showed that 28 cases were confirmed to be positive for antibodies to Lyme disease, which mainly included 31 KD outer surface protein A, 33 KD-36 KD outer surface protein B, 39 KDBorrelia burgdorferi membrane protein A,41 KD flagellin and 60 KD heat shock protein. Conclusions There exists Lyme disease infection among people in the northeast of Hainan province; and hence, local disease prevention and surveillance should be strengthened.
李沙, 张琳, 李欢, 侯学霞, 陈婷, 苗广青, 符晓莹, 陈海, 郝琴, 朱雄. 海南省东北部地区某类就诊患者莱姆病抗体检测结果分析[J]. 实用预防医学, 2020, 27(9): 1068-1071.
LI Sha, ZHANG Lin, LI Huan, HOU Xue-xia, CHEN Ting, MIAO Guang-qing, FU Xiao-ying, CHEN Hai, HAO Qin, ZHU Xiong. Test results for detection of antibody against Lyme disease in certain patients in the northeast of Hainan province. , 2020, 27(9): 1068-1071.
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2020, Vol. 27 
