Correlation between preS gene mutation of hepatitis B virus and liver cirrhosis after hepatitis B virus infection
XIE Jia-xin1, DING Yi-bo2, ZHANG Li1, WANG Fu-kun3, ZHANG Hai-pu2
1. The Third Military Medical University, Chongqing 400038, China; 2. The Second Military Medical University, Shanghai 2000433, China; 3. Bethune Hospital, Shijiazhuang, Hebei 050000, China
Abstract：Objective To explore the correlation of preS gene variation of hepatitis B virus (HBV) with the occurrence of liver cirrhosis (LC) after HBV infection. Methods A case-control study, including 50 patients with chronic hepatitis B (CHB) and 67 patients with LC after HBV infection, was designed. preS gene of serum HBV from all the subjects was amplified and sequenced. MEGA7 software was used to align the sequence. The correlation of mutations in HBV preS gene hot spots with LC was determined through univariate and multivariate analyses by using SPSS16.0 software. Results Univariate analysis indicated that HBV mutations in the preS region, including T3116m (χ2=8.470, P=0.004), A49m (χ2=4.939, P=0.026), T53m (χ2=6.683, P=0.010), A109m (χ2=5.868, P=0.015), and preS deletion (χ2=12.154, P=0.000) were significantly associated with the risks of LC. The frequency of preS deletion was significantly higher in decompensatory LC patients than in compensatory LC patients (63.16% vs. 31.03%, P=0.007). Multivariate analysis showed that advanced age (OR=1.07, 95%CI:1.02-1.11), T3116m (OR=4.18, 95%CI:1.39-12.61), and preS deletion (OR=7.20, 95%CI:2.09-24.80) were independent risk factors for LC. Conclusions HBV preS deletion and T3116m are the HBV mutations related to higher risk of progression from CHB to LC, which need to be validated in a larger sample size.
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2020, Vol. 27 
