Determination of free estrogens in beef samples by magnetic solid phaseextraction combined with high performance liquid chromatography
GUO Xun-yi1, YANG Zhen2, YAN Fu-hua2, YANG Sheng-yuan1, HU Cong2
1. School of Public Health, University of South China, Hengyang, Hunan 421001, China; 2. Changsha Municipal Center for Disease Control and Prevention, Changsha, Hunan 410004, China
Abstract：Objective To develop a new method for determination of free estrogens in beef by magnetic solid phase extraction (MSPE) coupled to high performance liquid chromatography-diode array detector (HPLC-DAD). Methods A new magnetic nanoparticle was prepared based on metal organic framework compounds, and served as MSPE adsorbent to establish a new method using HPLC-DAD for determination of 17 β-estradiol, estrone, 17α-ethinyl estradiol, diethylstilbestrol, dienestrol and bisphenol A. And the method was applied to analyzing the above-mentioned six estrogens in real beef samples. Results The detection limits of the method for the six estrogens were between 0.1 μg/L and 0.8 μg/L, and the liner ranges between 2-5,000 μg/L and 5-5,000 μg/L. The relative standard deviation of the method ranged from 4.9% to 10.5% (n=8), and the recoveries of standard addition for spiked beef samples were 79.1%-118%. Conclusions The new method possesses many advantages like low detection limit, wide liner range and strong anti-interference ability. It has a promising application prospect for the detection of estrogen in raw meat samples.
郭迅依, 杨珍, 严付华, 杨胜园, 胡骢. 磁固相萃取-高效液相色谱检测牛肉样品中的游离雌激素[J]. 实用预防医学, 2020, 27(4): 410-412.
GUO Xun-yi, YANG Zhen, YAN Fu-hua, YANG Sheng-yuan, HU Cong. Determination of free estrogens in beef samples by magnetic solid phaseextraction combined with high performance liquid chromatography. , 2020, 27(4): 410-412.
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2020, Vol. 27 
