Evolution rates of in vivo and horizontal transmission of HCV 1b subtype
XU Ru1,2, HUANG Jie-ting1,2, WANG Min1,2, LIAO Qiao1,2, RONG Xia1,2, FU Yong-shui1,2
1.Guangzhou Blood Center, Guanzhou, Guangdong 510095, China; 2.Guangzhou Key Laboratory of Medicine (Key Laboratory of Blood Safety), Guanzhou, Guangdong 510095, China
Abstract：Objective To obtain the evolution rates of in vivo and horizontal transmission of HCV 1b subtype and its biodiversity. Methods Samples collected from two blood donors infected with HCV in Guangdong region were followed up every 6 months. The next-generation sequencing technology and bioinformatics analysis method were used to obtain the whole mitochondrial genome sequence. Mega 7.0 software was employed to determine HCV genotype. The evolution rates of in vivo and horizontal transmission of HCV 1b subtype and its biodiversity were analyzed by using Markov Chain Monte Carlo (MCMC) method in Beast package. Results We obtained 6 HCV full-length sequences in three samples collected from two HCV-infected patients, with the average depth of 2,486. The 6 specimen sequences could basically cover the HCV whole genome, all of which were HCV 1b subtype. The evolution rate of in vivotransmission in the sample 1 was higher than that in the sample 2 under the relaxed lognormal model, while the evolution rates of in vivo transmission in samples 1 and 2 were both higher than those of horizontal transmission under relaxed lognormal and strict models. In addition, the recent common ancestor of HCV 1b in China originated in 1,938. The number of HCV-infected cases in China during 1986-1998 increased logarithmically, but there was a gradually decreasing trend after 1998. Conclusions The evolution rate of in vivo transmission of HCV 1b subtype is higher than that of horizontal transmission, and the evolution rates of different individuals are dissimilar.
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2020, Vol. 27 
