Detection of avian influenza virus in external environment of live poultry markets and serological surveillance results of occupationally exposed population in Guigang city, 2014-2018
LIANG Zheng-lin, YANG Zhong-xiu, DENG Xing-chao, LIN Yu-feng, HUANG Mei-ling
Guigang Municipal Center for Disease Control and Prevention, Guigang, Guangxi 537100, China
Abstract：Objective To investigate the positive rate and changing rules of avian influenza virus in live poultry trading markets and the serological monitoring results of people occupationally exposed to avian influenza virus in Guigang city from 2014 to 2018, and to provide a basis for prevention and control of human infection with avian influenza virus. Methods We collected 1,026 external environmental specimens from live poultry trading markets and poultry farmers markets and 231 serum specimens of occupationally exposed population in Guigang city during 2014-2018. Real-time fluorescent quantitative reverse transcription polymerase chain reaction was used to detect avian influenza virus nucleic acid in the external environmental specimens. And horse blood hemagglutination inhibition test was employed to detect H9N2, H7N9 and H5N6 antibodies in the serum specimens. Results The total positive rate of influenza A virus nucleic acid in the external environmental specimens from 2014 to 2018 was 37.62%, and the positive rate fluctuated between 13.89% and 52.63%, with an increasing trend year by year. Among the positive specimens, H9 subtype played a dominant role, with the positive number of 51.04%. The positive rate in the only one large-scale live poultry trading market in Guigang city was 22.57%, and H9 subtype was the main type. The positive rate in the farmer markets was 51.01%, H9 and H5 subtypes were the main types. Among the 5 different types of specimens, the positive rate in cleaning poultry sewage was the highest (53.67%). The positive rates in the first and fourth quarters were both higher than those in the second and third quarters. There were statistically significant differences in the positive detection rate of specimens among different years, specimen types, monitoring quarters, sites and virus typing (all P<0.05). The serological monitoring results of occupationally exposed population in 2014-2018 were all negative. There was only one case of human infection with avian influenza A (H5N6) virus among people with a history of exposure to poultry. Conclusions The detection rates of avian influenza virus nucleic acid in live poultry trading markets and farmers markets in Guigang city from 2014 to 2018 were relatively high, but the occupationally exposed people were generally not infected, indicating that the risk of human infection with avian influenza virus was relatively low. However, the high contamination rate of external environment and the occurrence of sporadic cases suggested there was still a risk of infection among general population. It is necessary to strengthen the supervision of markets, especially the monitoring in winter and spring, implementing prevention and control measures, such as disinfection of live poultry trading markets and standardized management, and simultaneously monitor influenza and unexplained pneumonia cases so as to maximumly decrease the risk of human infection with avian influenza virus.
梁正林, 杨忠秀, 邓星超, 林愈凤, 黄美玲. 2014—2018年贵港市活禽市场外环境禽流感病毒检测和职业暴露人群血清学监测结果分析[J]. 实用预防医学, 2020, 27(2): 138-141.
LIANG Zheng-lin, YANG Zhong-xiu, DENG Xing-chao, LIN Yu-feng, HUANG Mei-ling. Detection of avian influenza virus in external environment of live poultry markets and serological surveillance results of occupationally exposed population in Guigang city, 2014-2018. , 2020, 27(2): 138-141.
2020, Vol. 27 
