Experimental study on the effect of silkworm cocoon water extracts on immunomodulatory activity in mice
FU Wei-zhong1, QIN Hui-yan1, QIN Guang-qiu2, ZHANG Jie-hong1, LI Bin1, GAO Yu-qiu1, YAO Si-yu1, WEN Ping-jing1, WANG Yan-wu1, ZHAO Peng1
1. Guangxi Zhuang Autonomous Regional Center for Disease Control and Prevention, Nanning, Guangxi 530028, China;
2. Department of Toxicology and Sanitary Chemistry, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China
Abstract:Objective To explore the effect of silkworm cocoon water extracts (SCWEs) on immunomodulatory activity in mice. Methods Mice were treated with SCWEs by gastric intubation for 30 successive days at daily dosages of 1,000, 500 and 250 mg/kg.BW respectively, and their proliferation ability, delayed-type-hypersensitivity reaction, the level of serum hemolysin and number of hemolytic plaque, the abilities of carbon particle clearance and phagocytizing chicken red blood cells were detected. Results It was found that SCWE induced delayed-type-hypersensitivity in ears of mice. The differences in the average weight of ears in 1,000, 500 and 250 mg/kg treatment groups were (16.0±2.4), (15.9±3.0) and (14.3±2.6) respectively, which were higher than that of the negative control group (12.2±4.0). There were statistically significant differences in the average weight of ears between the negative control group and 1,000 and 500 mg/kg treatment groups (P<0.05). The average serum antibody counting in 1,000, 500 and 250 mg/kg treatment groups was (163.4±12.0), (150.4±13.4) and (148.4±14.2) respectively, which was higher than that of the negative control group ((141.6±11.5), P<0.01), with a statistically significant difference between 1,000 mg/kg treatment group and the negative control group (P<0.05). The average number of hemolytic plaque in 1,000, 500 and 250 mg/kg treatment groups was (217.4±26.4), (178.2±41.5) and (169.6±32.5) respectively, which was higher than that of the negative control group (162.4±38.2), with a statistically significant difference between 1,000 mg/kg treatment group and the negative control group (P<0.05). The ratios of peritoneal macrophage phagocytizing chicken red blood cells in 1,000, 500 and 250 mg/kg treatment groups were (28.2±4.57), (26.6±2.50) and (24.7±2.36) respectively, which were higher than that of the negative control group (23.3±3.40), with a statistically significant difference between the negative control group and 1,000 and 500 mg/kg treatment groups (P<0.05). The indexes of phagocytizing chicken red blood cells in 1,000, 500 and 250 mg/kg treatment groups were (0.625±0.134), (0.584±0.059) and (0.529±0.068) respectively, which were higher than that of the negative control group (0.490±0.086), with a statistically significant difference between the negative control group and 1,000 and 500 mg/kg treatment groups (P<0.05). Conclusions The results of this study suggest that SCWE has beneficial effects on immune function in mice under the conditions of the present study.
傅伟忠, 覃辉艳, 覃光球, 张洁宏, 李彬, 高玉秋, 姚思宇, 温平镜, 王彦武, 赵鹏. 缫丝水浓缩液对小鼠免疫功能影响的实验研究[J]. 实用预防医学, 2019, 26(6): 672-674.
FU Wei-zhong, QIN Hui-yan, QIN Guang-qiu, ZHANG Jie-hong, LI Bin, GAO Yu-qiu, YAO Si-yu, WEN Ping-jing, WANG Yan-wu, ZHAO Peng. Experimental study on the effect of silkworm cocoon water extracts on immunomodulatory activity in mice. , 2019, 26(6): 672-674.
[1] 范金波, 任发政, 孙雁, 等.丝胶蛋白抗氧化肽的酶法制备及功能评价[J].农业工程学报, 2009, 24(11):279-283.
[2] Chon JW, Lee KG, Yeo JH.Effect of silk fibroin hydrolysate on the apoptosis of MCF-7 human breast cancer cells [J].Int J Indust Entomol, 2013, 27(2):228-236.
[3] Park JH, Jung H, Nam H, et al.Silk fibroin hydrolysate ameliorates diabetic dyslipidemia in type 2 diabetic model mice [J].Anim Cell Syst, 2013, 17(5):325-330.
[4] 中华人民共和国卫生部.保健食品检验与评价技术规范[Z].2003.
[5] 李立, 王宁, 张桂霞, 等.蛋白质粉对小鼠免疫功能的影响[J].中国比较医学杂志, 2010, 20(3):54-56.
[6] 龚非力.医学免疫学[M].第2版.北京:科学出版社, 2004:171-174.
[7] Takasu Y, Yamada H, Tamura T, et al. Identification and characterization of a novel sericin gene expressed in the anterior middle silk gland of the silkworm Bombyx mori[J]. Insect Biochem Mol Biol,2007, 37:1234-1240.
[8] 周秀萍,李争鸣,刘志杰,等.不同方法制备金银花水提液对大鼠免疫功能影响的研究[J].实用预防医学,2011,18(11):2052-2054.
[9] Wang X, Qiao SY, Liu M, et al.Effects of graded levels of true ileal digestible threonine on performance ,serum parameters and immune function of 10-25 kg pigs [J] .Anim Feed Sci Tech, 2006, 129:264-278.
[10] 王斌.食品中的免疫调节功能因子研究进展[J].氨基酸和生物资源, 2009, 31(1):70-73.
[11] 周月婵,胡怡秀,马征,等.螺旋藻干粉制剂辅助降血脂和调节免疫力功能动物实验研究[J].实用预防医学,2006,13(2):300-303.
[12] 刘建亭, 王玉霞, 周晓潮, 等.家蚕茧壳水解物对小鼠免疫功能的影响[J].蚕业科学, 2015, 41(1):145-150.
[13] 王晶晶, 张泽峰, 邓红梅, 等.家蚕茧壳水解物对小鼠细胞免疫功能的影响[J].特产研究, 2016, 38(1):1-4.