Abstract：Objective To analyze the etiological features of 6 cases of suspected vaccine-associated measles in Zhongshan City. Methods Nucleic acids were extracted from throat swabs of 6 cases of suspected vaccine-associated measles in Zhongshan City from 2014 to 2015. 450 nucleotide sequences of the C-terminus of measles virus nucleoprotein (N) genes were amplified from the isolates using nested-polymerase chain reaction. Then the genetic relationship, homology of nucleotides, amino acids and amino acid sites of N gene among the deduced sequences, World Health Organization (WHO) reference strains and measles virus vaccine strains in China Shanghai191(S191) were analyzed. Results Among the 6 detected samples, 2 samples were negative in 450 nucleic acids. 4 samples were detected positive by RT-PCR, and sequence comparison showed that the identities of the nucleotide and the amino acid sequences among 3 viral strains were both 100%, and the homologies of nucleotide and amino acid sequences were both 100% as compared with S191 strain. The 3 viral strains and S191 strain both belonged to genotype A. Among the detected strains, one belonged to genotype H1a, and showed 97.7% and 96.6% identity respectively at nucleotide and amino acid sequence levels as compared with Chin9322-H1a strain. Conclusions Three cases were infected by the measles virus vaccine strain and one was infected with the wild strain.
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2019, Vol. 26 
