Virulence and antibiotic resistance characteristics and molecular typing of Staphylococcus aureus in samples from various sources in Shenzhen City, 2009-2015
YUAN Meng, HUANG Han-wei, HU Peng-wei, YUAN Yue-ming, LUO Jin-yan, ZHU Bing-qing
Nanshan District Center for Disease Control and Prevention, Shenzhen,Guangdong 518054,China
摘要目的 研究2009－2015年深圳市不同来源样本中金黄色葡萄球菌(Staphylococcus aureus,SA)携带毒力、耐药基因、耐药表型情况和分子型别研究。 方法 2009－2015年期间,86株SA分离自医院病人、医院环境涂抹样、食物中毒等样品。PCR方法检测毒力基因、耐药基因以及耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)的SCCmec基因型,进行蛋白A基因多态性(single locus DNA-sequencing of the repeat region of the Staphylococcus protein A gene,Spa)以及多位点序列分型(multilocus sequencing typing,MLST)研究。按照美国临床和实验室标准协会(Clinical and Laboratory Standards Institute,CLSI)方法,对抗菌药物进行药敏试验。 结果 检出21种毒力基因,55株(64.0%)携带mecA基因。SCCmec分型结果,Ⅲ型为优势型别(60.0%,33/55)。86株SA分为24种Spa型,MRSA菌株Spa优势型别为t030与t437,甲氧西林敏感金黄色葡萄球菌菌株(methicillin sensitive Staphylococcus aureus,MSSA)的优势型别为t091与t127。86株SA分为23种 MLST型,形成3个克隆复合体CC239(27.9%)、CCnew3(27.9%)与CC188(11.6%)。 结论 优势型别为ST239(Spa-t030)、STnew3(Spa-t437)具有多药耐药的特征,且携带多种毒力基因。MRSA流行株、非流行株与MSSA在携带毒力与耐药基因之间均存在差异。需要加强监测SA中的优势型别及MRSA菌株.关注其耐药的动态变化。
Abstract：Objective To study the virulence genes, drug-resistant genes, antibiotic resistance phenotype and molecular typing of Staphylococcus aureus (S. aureus) in samples from various sources in Shenzhen City during 2009-2015. Methods Eighty-six stains of S. aureus were isolated from specimens collected from hospital patients, nosocomial environment and food poisoning cases in Shenzhen City from 2009 to 2015. Polymerase chain reaction (PCR) assay was used to detect virulence genes, antibiotic resistant genes and staphylococcal cassette chromosome mec (SCCmec) types in methicillin-resistant Staphylococcus aureus (MRSA). Staphylococcus protein A gene (Spa) typing and multilocus sequencing typing (MLST) were performed. Antibiotic susceptibilities were determined with Kirby-Bauer disk diffusion method according to the criteria of Clinical & Laboratory Standards Institute (CLSI). Results Twenty-one virulence genes were detected, and 55(64.0%) strains carried mecA gene. SCCmec typing showed that type Ⅲ was the preponderant type (60.0%, 33/55). 24 Spa types were identified in the 86 strains of S. aureus. t030 and t437 were the predominant Spa types in MRSA strains, while t091 and t127 the predominant Spa types in methicillin sensitive Staphylococcus aureus (MSSA)strains. 23 different MLST sequence types were identified among the 86 strains of S. aureus. MLST analysis found 3 clonal complexes (CCs), including CC239(27.9%), CCnew3(27.9%) and CC188(11.6%). Conclusions ST239(Spa-t030) and STnew3 (Spa-t437) are the most prevalent Spa and MLST types among all the strains, which are resistant to many kinds of antibiotics and carry many virulence genes. There exist differences in carrying virulence and antibiotic resistance genes between epidemic/non-epidemic MRSA clones and MSSA isolates. It is necessary to intensify the supervision on the predominant Spa types in S. aureus strains and MRSA strains and pay attention to their dynamic changes in drug resistance.
袁梦, 黄汉伟, 胡鹏威, 袁月明, 罗锦雁, 朱兵清. 2009－2015年深圳市不同来源样本中金黄色葡萄球菌毒力耐药特征及分子分型研究[J]. 实用预防医学, 2019, 26(4): 420-425.
YUAN Meng, HUANG Han-wei, HU Peng-wei, YUAN Yue-ming, LUO Jin-yan, ZHU Bing-qing. Virulence and antibiotic resistance characteristics and molecular typing of Staphylococcus aureus in samples from various sources in Shenzhen City, 2009-2015. , 2019, 26(4): 420-425.
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