Abstract：Objective To develop a novel method for the determination of hippuric acid and methyl hippuric acid in urine by ultra high performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Methods We employed reversed phase T3 column and acetonitrile-methanol-water (with 0.1% formic acid) as elution solvent, and then detected hippuric acid and methyl hippuric acid in urine by electrospray ionization mass spectrometry. Results This method successfully realized the baseline separation and detection of hippuric acid and three isomers of methyl hippuric acid, and obtained low detection limits (0.07μg/L for hippuric acid and 0.1μg/L for methyl hippuric acid) and a wide liner range (1-10,000 μg/L). Conclusion Ultra high performance liquid chromatography-mass spectrometry has a promising application prospect in the determination of hippruic acid and methyl hippuric in urine.
杨珍, 李乐, 孙边成, 胡骢, 汪琼. 超高效液相色谱-质谱检测尿液中马尿酸和甲基马尿酸[J]. 实用预防医学, 2018, 25(12): 1422-1423.
YANG Zhen, LI Le, SUN Bian-cheng, HU Cong, WANG Qiong. Determination of hippuric acid and methyl hippuric acid in urine by ultra high performance liquid chromatography-mass spectrometry. , 2018, 25(12): 1422-1423.
[1] 中华人民共和国卫生部.WS/T 53-1996 尿中马尿酸、甲基马尿酸的高效液相色谱测定方法[S].北京:人民卫生出版社,1996:10-14. [2] Moein MM, EI-Beqqali A, Javanbakht M, et al.On-line detection of hippuric acid by microextraction with a molecularly-imprinted polysulfone membrane sorbent and liquid chromatography-tandem mass spectrometry[J].J Chromatogr A, 2014,1372:55-62. [3] Du Y, Li X,Zheng H, et al.Design of a calix[4]arene-functionalized metal-organic framework probe for highly sensitive and selective monitor of hippuric acid for indexing toluene exposure[J].Anal Chim Acta, 2018,1001:134-142. [4] Zhao F, Wang Z, Wang H, et al.Determination of hippuric acid in human urine by ion chromatography with conductivity detection[J].J Chromatogr B Analyt Technol Biomed Life Sci, 2011,879(3-4):296-298. [5] 肖润梅,孙敬智,梅勇,等.HPLC法同时测定尿样中苯、甲苯的4种代谢产物 [J].中国卫生检验杂志,2013,23(12):2559-2565. [6] 冯翠霞,张艳.高效液相色谱法测定尿中马尿酸、甲基马尿酸三种同分异构体 [J].广东化工,2010,37(7):147-148. [7] 卢丽明,黄诚.尿中马尿酸、甲基马尿酸的高效液相色谱测定法 [J].职业与健康,2008,24(17):1777-1778.
2018, Vol. 25 
