Abstract：Objective To study the apoptosis of human hepatoma HepG2 cells induced by dichloroacetonitrile (DCAN) and its related mechanism. Methods Dimethyl sulfoxide (DMSO)-treated HepG2 cells were selected as the control group, while 50 μmol/L and 800 μmol/L DCAN-treated HepG2 cells served as the DCAN-treated groups. The cell proliferation was detected with Cell Counting Kit-8 (CCK-8 ). Cell cycle and apoptosis rate were analyzed by flow cytometry with annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) double staining. The relative activity of caspase-3 in HepG2 cells was examined with fluorospectrophotometer assay. Results The interaction effect existed between DCAN concentration and treatment time (P<0.001). DCAN could significantly inhibit the proliferation of HepG2 cells in a dose- and time-dependent manner compared with the control group (P<0.05). The proportion of HepG2 cells in G0/G1 phase was decreased in the 800 μmol/L DCAN-treated group compared with the control group, while the proportion of HepG2 cells in G2/M phase was increased. Under the DCAN concentrations, the apoptosis rate of HepG2 cells were increased in a dose-dependent manner, with the regressionequation =0.031x+5 (R2=0.915, F=64.782, P<0.001), while the caspase 3 activity was also increased in a dose-dependent manner, with regression equation =0.0016x+1 (R2=1.000,F=21,266.064,P<0.001). Conclusions DCAN can inhibit the proliferation of HepG2 cells and induce their apoptosis, and the mechanism may be related to the arrest of G2/M phase, inhibition of RNA and protein synthesis and activation of caspase-3 pathway.
翟璐, 梁海荣, 罗皓. 二氯乙腈诱导HepG2细胞凋亡及机制研究[J]. 实用预防医学, 2018, 25(10): 1153-1155.
ZHAI Lu, LIANG Hai-rong, LUO Hao. Apoptosis of human hepatoma HepG2 cells induced by dichloroacetonitrile and its mechanism. , 2018, 25(10): 1153-1155.
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2018, Vol. 25 
